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Literature summary extracted from
- Characterization of oligosaccharides in recombinant tissue plasminogen activator produced in Chinese hamster ovary cells: two decades of analytical technology development (2009), Anal. Chem., 81, 9744-9754.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.21.68 | medicine | enzyme has important thrombolytic properties due to its high fibrin affinity, it is approved for treating acute myocardial infarction, or heart attacks, and later for acute ischemic stroke, and acute, massive pulmonary embolism | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.68 | rt-PA is produced by recombinant DNA technology in Chinese hamster ovary cell lines | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.68 | plasminogen + H2O | Homo sapiens | - |
plasminon + ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.68 | Homo sapiens | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.21.68 | glycoprotein | 3 major N-linked sites and one O-linked site are identified: site-specific N-glycosylation at typical Asn-Xaa-Ser/Thr motifs are due to asparagine glycosylation at sequence positions 117, 184, and 448, glycosylation at each individual site is heterogeneous: Asn117 glycosylation site contains high-mannose structures, whereas Asn184 and Asn448 are modified by complex/hybrid oligosaccharide structures. 2 further glycosylation site are identified in low abundance: the major oligosaccharides modifying Asn142 are biantennary monosialo and asialo hybrid and mannose-5-type structures, the other glycosylation site is at Thr61, elongation of O-fucose to bi-, tri-, and tetrasaccharides at Thr61 is identified at levels of about 0.7% | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.68 | plasminogen + H2O | - |
Homo sapiens | plasminon + ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.68 | rt-PA | - |
Homo sapiens |
| 3.4.21.68 | Tissue plasminogen activator | - |
Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.4.21.68 | physiological function | enzyme is a serine protease | Homo sapiens |
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Release 2023.1 (January 2023)
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