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Literature summary extracted from

  • Pungor, E.; Hague, C.M.; Chen, G.; Lemontt, J.F.; Dvorak-Ewell, M.; Prince, W.S.
    Development of a functional bioassay for arylsulfatase B using the natural substrates of the enzyme (2009), Anal. Biochem., 395, 144-150.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.6.12 expression of ASB C53S mutant in CHO cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.6.12 C53S the mutation causes ASB-deficiency, phenoytpe, overview. The enzyme is taken up into cultured ASB-deficient human fibroblasts, GM00519 cells, and translocates to the lysosomes, it is catalytically active. The enzyme enters target cells predominantly through the CI-M6P receptor. The uptake of rhASB is able to restore lysosomal function in an in vitro cell-based assay Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.6.12 lysosome
-
 Homo sapiens 5764
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.12 chondroitin sulfate + H2O Homo sapiens
-
 ?
-
 ?
3.1.6.12 dermatan sulfate + H2O Homo sapiens
-
 ?
-
 ?
3.1.6.12 additional information Homo sapiens ASB is a lysosomal exohydrolase, cleaving the 4-sulfate from the N-acetylgalactosamine-4-sulfate residue at the nonreducing terminal of glycosaminoglycan structures ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.12 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.12 fibroblast
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.12 chondroitin sulfate + H2O
-
 Homo sapiens ?
-
 ?
3.1.6.12 dermatan sulfate + H2O
-
 Homo sapiens ?
-
 ?
3.1.6.12 additional information ASB is a lysosomal exohydrolase, cleaving the 4-sulfate from the N-acetylgalactosamine-4-sulfate residue at the nonreducing terminal of glycosaminoglycan structures Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.6.12 arylsulfatase B
-
 Homo sapiens
3.1.6.12 ASB
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.6.12 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.12 8.2
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.1.6.12 malfunction ASB-deficient cells accumulate dermatan sulfate and chondroitin sulfate, which may be partially hydrolyzed by other lysosomal hydrolases Homo sapiens