Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nakayama, D.; Ben Ammar, Y.; Takeda, S.
    Crystallization and preliminary X-ray crystallographic analysis of blood coagulation factor V-activating proteinase (RVV-V) from Russell’s viper venom (2009), Acta Crystallogr. Sect. F, 65, 1306-1308.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.95 in complex with Pefabloc or D-Phe-Pro-Arg-chloromethylketone, sitting drop vapor diffusion method, using 0.8% (w/v) tryptone, 0.04 M Na HEPES pH 7.0 and 9.6% (w/v) PEG 3350 Daboia siamensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.95 D-Phe-Pro-Arg-chloromethylketone
-
 Daboia siamensis
3.4.21.95 pefabloc 4-(2-aminoethyl)-benzenesulfonyl fluoride Daboia siamensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.95 29000
-
 SDS-PAGE Daboia siamensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.95 Daboia siamensis
-
 Russell's viper
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.95 Resource S column chromatography and Sephacryl S-100 gel filtration Daboia siamensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.95 venom
-
 Daboia siamensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.95 factor V + H2O specifically activates factor V by cleaving a single peptide bond between Arg1545 and Ser1546 Daboia siamensis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.95 blood coagulation factor V-activating proteinase
-
 Daboia siamensis
3.4.21.95 RVV-V
-
 Daboia siamensis