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Literature summary extracted from

  • Baranowska-Bosiacka, I.; Dziedziejko, V.; Safranow, K.; Gutowska, I.; Marchlewicz, M.; Dolegowska, B.; Rac, M.E.; Wiszniewska, B.; Chlubek, D.
    Inhibition of erythrocyte phosphoribosyltransferases (APRT and HPRT) by Pb2+: a potential mechanism of lead toxicity (2009), Toxicology, 259, 77-83.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.7 Pb2+ inhibits the enzyme in erythrocytes about 25% at 0.0005 mM and about 20% at 0.0001 mM, and participates in hemolysis, the intensity of which negatively correlates with the activity of phosphoribosyltransferases, APRT inhibition as one of the mechanisms of lead toxicity Homo sapiens
2.4.2.7 Pb2+ moderately inhibits both the enzyme in erythrocytes even at very low concentrations, and participates in hemolysis, the intensity of which negatively correlates with the activity of phosphoribosyltransferases, APRT inhibition as one of the mechanisms of lead toxicity Rattus norvegicus
2.4.2.8 Pb2+ inhibits the enzyme in erythrocytes about 20% at 0.0005 mM and about 12% at 0.0001 mM, and participates in hemolysis, the intensity of which negatively correlates with the activity of phosphoribosyltransferases, HPRT inhibition as one of the mechanisms of lead toxicity Homo sapiens
2.4.2.8 Pb2+ moderately inhibits both the enzyme in erythrocytes even at very low concentrations, and participates in hemolysis, the intensity of which negatively correlates with the activity of phosphoribosyltransferases, HPRT inhibition as one of the mechanisms of lead toxicity Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.7 Mg2+
-
 Homo sapiens
2.4.2.7 Mg2+
-
 Rattus norvegicus
2.4.2.8 Mg2+
-
 Homo sapiens
2.4.2.8 Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Homo sapiens
-
 AMP + diphosphate
-
 ?
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Rattus norvegicus
-
 AMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Homo sapiens
-
 IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Rattus norvegicus
-
 IMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Homo sapiens
-
-
-
2.4.2.7 Rattus norvegicus
-
-
-
2.4.2.8 Homo sapiens
-
-
-
2.4.2.8 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.7 erythrocyte
-
 Homo sapiens
-
2.4.2.7 erythrocyte
-
 Rattus norvegicus
-
2.4.2.8 erythrocyte
-
 Homo sapiens
-
2.4.2.8 erythrocyte
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.7 additional information
-
 enzyme activity in erythrocyte lysate in presence or absence of Pb2+, overview Homo sapiens
2.4.2.7 additional information
-
 enzyme activity in erythrocyte lysate in presence or absence of Pb2+, overview Rattus norvegicus
2.4.2.8 additional information
-
 enzyme activity in erythrocyte lysate in presence or absence of Pb2+, overview Homo sapiens
2.4.2.8 additional information
-
 enzyme activity in erythrocyte lysate in presence or absence of Pb2+, overview Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens AMP + diphosphate
-
 ?
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Rattus norvegicus AMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Rattus norvegicus IMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.7 APRT
-
 Homo sapiens
2.4.2.7 APRT
-
 Rattus norvegicus
2.4.2.8 HPRT
-
 Homo sapiens
2.4.2.8 HPRT
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.7 37
-
 assay at Homo sapiens
2.4.2.7 37
-
 assay at Rattus norvegicus
2.4.2.8 37
-
 assay at Homo sapiens
2.4.2.8 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.7 7.4
-
 assay at Homo sapiens
2.4.2.7 7.4
-
 assay at Rattus norvegicus
2.4.2.8 7.4
-
 assay at Homo sapiens
2.4.2.8 7.4
-
 assay at Rattus norvegicus

General Information

EC Number General Information Comment Organism
2.4.2.7 physiological function the enzyme enables the reutilization of purine base adenine converting it to mononucleotide AMP, substrate for the synthesis of high-energy nucleotides Homo sapiens
2.4.2.7 physiological function the enzyme enables the reutilization of purine base adenine converting it to mononucleotide AMP, substrate for the synthesis of high-energy nucleotides Rattus norvegicus
2.4.2.8 physiological function the enzyme enables the reutilization of purine base adenine converting it to mononucleotide AMP, substrate for the synthesis of high-energy nucleotides Homo sapiens
2.4.2.8 physiological function the enzyme enables the reutilization of purine base adenine converting it to mononucleotide AMP, substrate for the synthesis of high-energy nucleotides Rattus norvegicus