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Literature summary extracted from

  • Andringa, K.K.; Bajt, M.L.; Jaeschke, H.; Bailey, S.M.
    Mitochondrial protein thiol modifications in acetaminophen hepatotoxicity: effect on HMG-CoA synthase (2008), Toxicol. Lett., 177, 188-197.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.3.10 medicine acetaminophen, APAP, is a leading cause of drug-induced liver failure Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.10 mitochondrion
-
 Mus musculus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.10 56800
-
 determined by MALDI-TOF mass spectrometry Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + H2O + acetoacetyl-CoA Mus musculus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Mus musculus P54869
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10 mitochondria are prepared Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.10 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.10 0.035
-
 activity in response to acetaminophen, APAP, treatment Mus musculus
2.3.3.10 0.057
-
 control, no acetaminophen, APAP, treatment Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + H2O + acetoacetyl-CoA
-
 Mus musculus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.3.3.10 3-hydroxy-3-methylglutaryl-CoA synthase 2
-
 Mus musculus
2.3.3.10 HMG-CoA synthase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.10 30
-
 activity assay Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.10 8.2
-
 activity assay Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.3.10 CoA
-
 Mus musculus

Expression

EC Number Organism Comment Expression
2.3.3.10 Mus musculus 3-hydroxy-3-methylglutaryl-CoA synthase 2 has significantly decreased levels of reduced thiols and activity after acetaminophen treatment down