EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.84 | expressed in Escherichia coli BL21(DE3) cells | Rhodopseudomonas palustris |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.84 | additional information | Rhodopseudomonas palustris | the nitrile hydratase can hydrate aliphatic, aromatic and heterocyclic nitriles under very mild conditions, in mixtures of pH 7 buffer and a range of organic solvents, often with excellent chemoselectivity. Major determinant of hydration occurring is the degree of steric hindrance around the nitrile moiety and/or size of the substrates | ? | - |
? | |
4.2.1.84 | additional information | Rhodopseudomonas palustris CGA009 | the nitrile hydratase can hydrate aliphatic, aromatic and heterocyclic nitriles under very mild conditions, in mixtures of pH 7 buffer and a range of organic solvents, often with excellent chemoselectivity. Major determinant of hydration occurring is the degree of steric hindrance around the nitrile moiety and/or size of the substrates | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.84 | Rhodopseudomonas palustris | - |
- |
- |
4.2.1.84 | Rhodopseudomonas palustris CGA009 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.84 | 2,6-difluorobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 2,6-difluorobenzamide | - |
? | |
4.2.1.84 | 2,6-difluorobenzonitrile + H2O | - |
Rhodopseudomonas palustris CGA009 | 2,6-difluorobenzamide | - |
? | |
4.2.1.84 | 2-bromobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 2-bromobenzamide | - |
ir | |
4.2.1.84 | 2-chlorobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 2-chlorobenzamide | - |
ir | |
4.2.1.84 | 2-fluorobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 2-fluorobenzamide | - |
ir | |
4.2.1.84 | 2-methoxybenzonitrile + H2O | - |
Rhodopseudomonas palustris | 2-methoxybenzamide | - |
ir | |
4.2.1.84 | 2-methylbenzonitrile + H2O | - |
Rhodopseudomonas palustris | 2-methylbenzamide | - |
ir | |
4.2.1.84 | 3-(trifluoromethyl)benzonitrile + H2O | 5% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-(trifluoromethyl)benzamide | - |
? | |
4.2.1.84 | 3-(trifluoromethyl)benzonitrile + H2O | 5% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris CGA009 | 3-(trifluoromethyl)benzamide | - |
? | |
4.2.1.84 | 3-(trifluoromethyl)pyridine-4-carbonitrile + H2O | - |
Rhodopseudomonas palustris | 3-(trifluoromethyl)pyridine-4-carboxamide | - |
ir | |
4.2.1.84 | 3-bromobenzonitrile + H2O | 5% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-bromobenzamide | - |
? | |
4.2.1.84 | 3-chlorobenzonitrile + H2O | 95% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-chlorobenzamide | - |
? | |
4.2.1.84 | 3-fluorobenzonitrile + H2O | above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-fluorobenzamide | - |
? | |
4.2.1.84 | 3-methoxybenzonitrile + H2O | 3% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-methoxybenzamide | - |
? | |
4.2.1.84 | 3-methylbenzonitrile + H2O | 17% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-methylbenzamide | - |
? | |
4.2.1.84 | 3-phenylpropanenitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 3-phenylpropanamide | - |
ir | |
4.2.1.84 | 4-(trifluoromethyl)benzonitrile + H2O | above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-(trifluoromethyl)benzamide | - |
ir | |
4.2.1.84 | 4-acetylbenzonitrile + H2O | above 99% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-acetylbenzamide | - |
ir | |
4.2.1.84 | 4-bromobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 4-bromobenzamide | - |
ir | |
4.2.1.84 | 4-bromobenzonitrile + H2O | above 99% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-bromobenzamide | - |
? | |
4.2.1.84 | 4-chlorobenzonitrile + H2O | above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-chlorobenzamide | - |
? | |
4.2.1.84 | 4-chlorobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 4-chlorbenzamide | - |
ir | |
4.2.1.84 | 4-fluorobenzonitrile + H2O | above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-fluorobenzamide | - |
? | |
4.2.1.84 | 4-fluorobenzonitrile + H2O | - |
Rhodopseudomonas palustris | 4-fluorbenzamide | - |
ir | |
4.2.1.84 | 4-hydroxybenzonitrile + H2O | - |
Rhodopseudomonas palustris | 4-hydroxybenzoic acid amide | - |
? | |
4.2.1.84 | 4-hydroxybenzonitrile + H2O | - |
Rhodopseudomonas palustris CGA009 | 4-hydroxybenzoic acid amide | - |
? | |
4.2.1.84 | 4-hydroxybenzonitrile + H2O | - |
Rhodopseudomonas palustris | 4-hydroxybenzamide | - |
? | |
4.2.1.84 | 4-methoxybenzonitrile + H2O | above 99% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-methoxybenzamide | - |
ir | |
4.2.1.84 | 4-methylbenzonitrile + H2O | above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 4-methylbenzamide | - |
ir | |
4.2.1.84 | benzonitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | benzamide | - |
ir | |
4.2.1.84 | benzonitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris CGA009 | benzamide | - |
ir | |
4.2.1.84 | ethyl 2-cyanobenzoate + H2O | - |
Rhodopseudomonas palustris | ethyl 2-carbamoylbenzoate | - |
ir | |
4.2.1.84 | ethyl 3-cyanobenzoate + H2O | no conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | ethyl 3-carbamoylbenzoate | - |
? | |
4.2.1.84 | ethyl 4-cyanobenzoate + H2O | 95% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | ethyl 4-carbamoylbenzoate | - |
ir | |
4.2.1.84 | furan-2-carbonitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | furan-2-carboxamide | - |
ir | |
4.2.1.84 | methyl 4-cyanobenzoate + H2O | 98% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | methyl 4-carbamoylbenzoate | - |
ir | |
4.2.1.84 | additional information | the nitrile hydratase can hydrate aliphatic, aromatic and heterocyclic nitriles under very mild conditions, in mixtures of pH 7 buffer and a range of organic solvents, often with excellent chemoselectivity. Major determinant of hydration occurring is the degree of steric hindrance around the nitrile moiety and/or size of the substrates | Rhodopseudomonas palustris | ? | - |
? | |
4.2.1.84 | additional information | the nitrile hydratase can hydrate aliphatic, aromatic and heterocyclic nitriles under very mild conditions, in mixtures of pH 7 buffer and a range of organic solvents, often with excellent chemoselectivity. Major determinant of hydration occurring is the degree of steric hindrance around the nitrile moiety and/or size of the substrates | Rhodopseudomonas palustris CGA009 | ? | - |
? | |
4.2.1.84 | phenylacetonitrile + H2O | 28% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 2-phenylacetamide | - |
ir | |
4.2.1.84 | pyridine-2-carbonitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | pyridine-2-carboxamide | - |
ir | |
4.2.1.84 | pyridine-4-carbonitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | pyridine-4-carboxamide | - |
ir | |
4.2.1.84 | thiophen-2-ylacetonitrile + H2O | 89% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | 2-(thiophen-2-yl)acetamide | - |
ir | |
4.2.1.84 | thiophene-2-carbonitrile + H2O | 99.9% conversion, in phosphate buffer pH 7.0, at 30°C | Rhodopseudomonas palustris | thiophene-2-carboxamide | - |
? | |
4.2.1.84 | thiophene-2-carbonitrile + H2O | - |
Rhodopseudomonas palustris | thiophen-2-carboxamide | - |
ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.84 | NHase | - |
Rhodopseudomonas palustris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.84 | 30 | - |
assay at | Rhodopseudomonas palustris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.84 | 7 | - |
assay at | Rhodopseudomonas palustris |