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Literature summary extracted from

  • Basturea, G.N.; Deutscher, M.P.
    Substrate specificity and properties of the Escherichia coli 16S rRNA methyltransferase, RsmE (2007), RNA, 13, 1969-1976.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.193 spermidine in the presence of spermidine, Mg2+ is not required for activity. Activity could be restored up to 60% of that with Mg2+ by addition of spermidine at 30 mM Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.193 EDTA 10 mM, complete inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.193 0.002
-
 uracil1498 in 16S rRNA pH 7.0, 37°C, the Km-value for 16S rRNA is measured as a Km for the physiological substrate, 30S ribosomal subunit Escherichia coli
2.1.1.193 0.0267
-
 S-adenosyl-L-methionine pH 7.0, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.193 Mg2+ most active in the presence of 10–15 mM Mg2+ at pH 7–9. At 30 mM Mg2+ optimal activity is reduced by 60%. In the presence of spermidine, Mg2+ is not required for activity. Activity could be restored up to 60% of that with Mg2+ by addition of spermidine at 30 mM Escherichia coli
2.1.1.193 NH4+ most active in the presence of 100 mM NH4Cl at pH 7–9 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.193 50000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.193 S-adenosyl-L-methionine + uracil1498 in 16S rRNA Escherichia coli activity is dependent on a fully assembled 30 S ribosome subunit S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.193 Escherichia coli P0AGL7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.193 S-adenosyl-L-methionine + uracil1498 in 16S rRNA activity is dependent on a fully assembled 30 S ribosome subunit Escherichia coli S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
 ?
2.1.1.193 S-adenosyl-L-methionine + uracil1498 in 16S rRNA small ribosome subunits obtained from an RsmE deletion strain can be methylated by purified RsmE, neither 70S ribosomes nor 50S subunits are active. 16S rRNA obtained from the mutant strain, synthetic 16S rRNA, and 3' minor domain RNA are all very poor or inactive as substrates. 30S particles partially depleted of proteins by treatment with high concentrations of LiCl or in vitro reconstituted intermediate particles also show little or no methyl acceptor activity. RsmE requires a highly structured ribonucleoprotein particle as a substrate for methylation Escherichia coli S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.193 dimer forms dimers in solution Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.1.1.193 RsmE
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.193 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.193 0.0007
-
 S-adenosyl-L-methionine pH 7.0, 37°C Escherichia coli
2.1.1.193 0.0013
-
 uracil1498 in 16S rRNA pH 7.0, 37°C, the Km-value for 16S rRNA is measured as a Km for the physiological substrate, 30S ribosomal subunit Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.193 7
-
 assay at Escherichia coli