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Literature summary extracted from

  • Song, H.N.; Jung, T.Y.; Park, J.T.; Park, B.C.; Myung, P.K.; Boos, W.; Woo, E.J.; Park, K.H.
    Structural rationale for the short branched substrate specificity of the glycogen debranching enzyme GlgX (2010), Proteins, 78, 1847-1855.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.196 expressed in Escherichia coli MC1061 cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.196 hanging drop vapor diffusion method, using 0.2 M sodium citrate, 47% (v/v) MPD, 4% (v/v) PEG 3350, and HEPES (pH 8.0) buffer Escherichia coli
3.2.1.196 hanging drop vapor diffusion method, using 0.2 M sodium citrate, 47% (w/v) 2-methyl-2,4-pentanediol, 4% (w/v) PEG 3350, and HEPES (pH 8.0) buffer Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.196 0.15
-
 G4-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH7.0) at 37°C Escherichia coli
3.2.1.196 1.51
-
 G3-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH7.0) at 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.196 phosphorylase-limit dextrin + H2O Escherichia coli the enzyme hydrolyzes alpha-1,6-glycosidic linkages of phosphorylase-limit dextrin containing only three or four glucose subunits ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.196 Escherichia coli
-
-
-
3.2.1.196 Escherichia coli P15067
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.196 Ni-NTA column chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.196 G3-beta-cyclodextrin + H2O
-
 Escherichia coli ?
-
 ?
3.2.1.196 G4-beta-cyclodextrin + H2O
-
 Escherichia coli ?
-
 ?
3.2.1.196 G4-beta-cyclodextrin + H2O GlgX shows substrate specificity for G4 phosphorylase-limit dextrin Escherichia coli ?
-
 ?
3.2.1.196 additional information no activity towards G2-beta-cyclodextrin and G5-beta-cyclodextrin Escherichia coli ?
-
 ?
3.2.1.196 additional information no activity with G2- and G5-beta-cyclodextrin Escherichia coli ?
-
 ?
3.2.1.196 phosphorylase-limit dextrin + H2O the enzyme hydrolyzes alpha-1,6-glycosidic linkages of phosphorylase-limit dextrin containing only three or four glucose subunits Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.196 GDE
-
 Escherichia coli
3.2.1.196 GlgX
-
 Escherichia coli
3.2.1.196 glycogen debranching enzyme
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.196 38
-
 G3-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH7.0) at 37°C Escherichia coli
3.2.1.196 41.7
-
 G4-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH7.0) at 37°C Escherichia coli