Literature summary extracted from

Misra, G.; Aggarwal, A.; Dube, D.; Zaman, M.S.; Singh, Y.; Ramachandran, R.
Crystal structure of the Bacillus anthracis nucleoside diphosphate kinase and its characterization reveals an enzyme adapted to perform under stress conditions (2009), Proteins, 76, 496-506.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.7.4.6	expressed in Escherichia coli BL21(DE3) cells	Bacillus anthracis

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.7.4.6	-	Bacillus anthracis

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.7.4.6	G19V	the mutation rigidifies the 43-63 loop in Ndk, using GTP as the phosphate donor a 300fold reduction in phosphotransferase activity is observed	Bacillus anthracis
2.7.4.6	H115Q	H115Q is incompetent in autophosphorylation, confirming the role of histidine as a phosphate acceptor	Bacillus anthracis

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.7.4.6	EDTA	complete inhibition at 10 mM	Bacillus anthracis

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.7.4.6	Mg2+	the activity is dependent on a metal ion (mainly Mg2+)	Bacillus anthracis

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.7.4.6	ATP + NDP	Bacillus anthracis	-	ADP + NTP	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.4.6	Bacillus anthracis	Q81SV8	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
2.7.4.6	phosphoprotein	the presence of diethyl dicarbonate inhibits the autophosphorylation of Ndk	Bacillus anthracis

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.4.6	Ni-NTA column chromatography	Bacillus anthracis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.4.6	ATP + GDP	-	Bacillus anthracis	ADP + GTP	-	?
2.7.4.6	ATP + NDP	-	Bacillus anthracis	ADP + NTP	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.7.4.6	hexamer	exists as a hexamer that is composed of two SDS-stable trimers interacting in a back-to-back association	Bacillus anthracis

Synonyms

EC Number	Synonyms	Comment	Organism
2.7.4.6	NDK	-	Bacillus anthracis
2.7.4.6	nucleoside diphosphate kinase	-	Bacillus anthracis

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
2.7.4.6	10	95	-	Bacillus anthracis

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
2.7.4.6	3.5	10.5	-	Bacillus anthracis

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
2.7.4.6	ATP	-	Bacillus anthracis

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Release 2023.1 (January 2023)