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Literature summary extracted from
- Expression, purification and characterization of the acyl carrier protein phosphodiesterase from Pseudomonas aeruginosa (2010), Protein Expr. Purif., 71, 132-138.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.14 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.14 | 0.4 | - |
bis-4-nitrophenyl phosphate | - |
Pseudomonas aeruginosa |  |
| 3.1.4.14 | 1 | - |
2',3'-cAMP | - |
Pseudomonas aeruginosa | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.14 | Pseudomonas aeruginosa | - |
- |
- |
| 3.1.4.14 | Pseudomonas aeruginosa PAO-1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.14 | Ni2+-NTA resin column chromatography and Superdex-75 gel filtration | Pseudomonas aeruginosa |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.1.4.14 | - |
Pseudomonas aeruginosa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.4.14 | AcpH is stable enough that it can be concentrated and stored at concentrations up to 1 mg/ml without causing severe aggregation | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.14 | 2',3'-cAMP + H2O | - |
Pseudomonas aeruginosa | 3'-AMP | - |
? | |
| 3.1.4.14 | 2',3'-cAMP + H2O | - |
Pseudomonas aeruginosa PAO-1 | 3'-AMP | - |
? | |
| 3.1.4.14 | acyl-carrier protein + H2O | acyl-carrier protein holo-PA2966 is converted completely back to its apo-form within 40 min, whereas the acyl-carrier proteins PA1869 and PA4226 remain in its holo form even after prolonged incubation. Partial conversion is observed for acyl-carrier protein PA3334 with both the apo and holo forms present after the incubation | Pseudomonas aeruginosa | 4'-phosphopantetheine + apoprotein | - |
? | |
| 3.1.4.14 | acyl-carrier protein + H2O | acyl-carrier protein holo-PA2966 is converted completely back to its apo-form within 40 min, whereas the acyl-carrier proteins PA1869 and PA4226 remain in its holo form even after prolonged incubation. Partial conversion is observed for acyl-carrier protein PA3334 with both the apo and holo forms present after the incubation | Pseudomonas aeruginosa PAO-1 | 4'-phosphopantetheine + apoprotein | - |
? | |
| 3.1.4.14 | acyl-carrier protein domain of the iterative polyketide synthase in calicheamicin biosynthesis + H2O | partial conversion | Pseudomonas aeruginosa | ? | - |
? | |
| 3.1.4.14 | acyl-carrier protein domain of the iterative polyketide synthase in calicheamicin biosynthesis + H2O | partial conversion | Pseudomonas aeruginosa PAO-1 | ? | - |
? | |
| 3.1.4.14 | acyl-carrier protein from Escherichia coli fatty acid synthesis pathway + H2O | complete conversion | Pseudomonas aeruginosa | ? | - |
? | |
| 3.1.4.14 | bis-4-nitrophenyl phosphate + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.1.4.14 | CalE8 + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.1.4.14 | additional information | AcpH does not hydrolyze 3',5'-cAMP | Pseudomonas aeruginosa | ? | - |
? | |
| 3.1.4.14 | additional information | acyl-carrier protein domain from the modular polyketide synthase DEBS is a poor substrate for AcpH | Pseudomonas aeruginosa | ? | - |
? | |
| 3.1.4.14 | additional information | AcpH does not hydrolyze 3',5'-cAMP | Pseudomonas aeruginosa PAO-1 | ? | - |
? | |
| 3.1.4.14 | additional information | acyl-carrier protein domain from the modular polyketide synthase DEBS is a poor substrate for AcpH | Pseudomonas aeruginosa PAO-1 | ? | - |
? | |
| 3.1.4.14 | peptidyl carrier protein domain of non-ribosomal peptide synthase + H2O | complete conversion | Pseudomonas aeruginosa | ? | - |
? | |
| 3.1.4.14 | thymidine 5-monophosphate 4-nitrophenyl ester + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.14 | monomer | about half of the soluble fraction of AcpH is obtained as monomeric protein | Pseudomonas aeruginosa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.14 | AcpH | - |
Pseudomonas aeruginosa |
| 3.1.4.14 | acyl carrier protein phosphodiesterase | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.14 | 0.004 | - |
2',3'-cAMP | - |
Pseudomonas aeruginosa | |
| 3.1.4.14 | 0.21 | - |
bis-4-nitrophenyl phosphate | - |
Pseudomonas aeruginosa | |
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