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Literature summary extracted from

  • Cheng, C.Y.; Yu, Y.J.; Yang, M.T.
    Coexpression of omega subunit in E. coli is required for the maintenance of enzymatic activity of Xanthomonas campestris pv. campestris RNA polymerase (2010), Protein Expr. Purif., 69, 91-98.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.6 expression of C-terminally His-tagged alpha subunit-encoded by gene rpoA, and coexpression of the subunits of Xanthomonas campestris pv. campestris core RNAP from plasmid pBBad22K in Escherichia coli strain BL21(DE3), overexpression with or without omega Xanthomonas campestris

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.6 additional information recombinant Xcc core RNAP lacking omega shows a 13fold decrease in enzymatic activity in comparison with that containing omega. Promoter-specific transcription assays by recombinant Xcc core RNAP reconstituted with external added sigma factor show that the absence of omega debilitates the transcriptional activity of Xcc RNAP Xanthomonas campestris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 Mg2+
-
 Xanthomonas campestris

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Xanthomonas campestris O08348 rpoD; pv. campestris
-
2.7.7.6 Xanthomonas campestris P0A0Y1 rpoA, alpha-subunit; pv. campestris
-
2.7.7.6 Xanthomonas campestris Q8PC55 rpoC; pv. campestris
-
2.7.7.6 Xanthomonas campestris Q8PC56 rpoB; pv. campestris
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6 recombinant Xcc core RNAP subunits from Escherichia coli by a two-step chromatographic process Xanthomonas campestris

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.7.6 assembly of Xcc core RNAP subunits recombinantly coexpressed in Escherichia coli after purification by a two-step chromatographic process Xanthomonas campestris
2.7.7.6 assembly of Xcc core RNAP subunits recombinantly coexpressed in Escherichia coli after purification by a two-step chromatographic process including nickel affinity chromatography Xanthomonas campestris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.6 additional information
-
 recombinant Xcc core RNAP lacking omega shows a 13fold decrease in enzymatic activity in comparison with that containing omega Xanthomonas campestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 additional information in vitro transcriptional activity of recombinant assembled Xcc RNAP, overview Xanthomonas campestris ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.6 RNA polymerase
-
 Xanthomonas campestris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.6 28
-
 assay at Xanthomonas campestris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.6 7.9
-
 assay at Xanthomonas campestris