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Literature summary extracted from

  • Lai, X.; Wu, J.; Chen, S.; Zhang, X.; Wang, H.
    Expression, purification, and characterization of a functionally active Mycobacterium tuberculosis UDP-glucose pyrophosphorylase (2008), Protein Expr. Purif., 61, 50-56.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.9 His-tagged version expressed in Escherichia coli BL21(DE3) Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 0.01187
-
 UTP
-
 Mycobacterium tuberculosis
2.7.7.9 0.04455
-
 alpha-D-glucose 1-phosphate
-
 Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.9 cell wall
-
 Mycobacterium tuberculosis 5618
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.9 Mg2+ absolutely required Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.9 36000
-
 monomer and different oligomers, gel filtration, x * 36000, SDS-PAGE, x * 35827.0, MALDI-TOF (calculated: 35938.8) Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Mycobacterium tuberculosis O05576
-
-
2.7.7.9 Mycobacterium tuberculosis H37Rv O05576
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9 immobilized metal affinity chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.9 0.1005
-
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Mycobacterium tuberculosis diphosphate + UDP-glucose
-
 r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Mycobacterium tuberculosis H37Rv diphosphate + UDP-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.9 monomer monomer and different oligomers, gel filtration, x * 36000, SDS-PAGE, x * 35827.0, MALDI-TOF (calculated: 35938.8) Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.7.9 UDP-glucose pyrophosphorylase
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.9 50
-
 10 min treatment: about 15% activity Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.9 55.03
-
 alpha-D-glucose 1-phosphate
-
 Mycobacterium tuberculosis
2.7.7.9 93.44
-
 UTP
-
 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.9 8
-
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.7.7.9 metabolism involved in cell envelope precursor synthesis Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.9 1240
-
 alpha-D-glucose 1-phosphate
-
 Mycobacterium tuberculosis
2.7.7.9 7870
-
 UTP
-
 Mycobacterium tuberculosis