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Literature summary extracted from

  • Lee, H.; McKeon, R.J.; Bellamkonda, R.V.
    Sustained delivery of thermostabilized chABC enhances axonal sprouting and functional recovery after spinal cord injury (2010), Proc. Natl. Acad. Sci. USA, 107, 3340-3345.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.2.20 medicine development of thermostabilized chondroitinase ABC and a system for its sustained local delivery in vivo, obviating the need for chronically implanted catheters and pumps. Presence of with 1M trehalose significantly enhances chondroitinase ABC thermal stability and prolongs enzyme activity. Thermostabilized chondroitinase ABC remains active at 37°C in vitro for up to 4 weeks. Chondroitin sulfate proteoglycan levels remain low in vivo up to 6 weeks post-spinal cord injury when thermostabilized chondroitin sulfate proteoglycan ABC is delivered by a hydrogel-microtube scaffold system. Axonal growth and functional recovery following the sustained local release of thermostabilized chondroitinase ABC versus a single treatment of unstabilized chondroitinase ABC demonstrate significant differences in chondroitin sulfate proteoglycan digestion. Animals treated with thermostabilized chondroitinase ABC in combination with sustained neurotrophin-3 delivery show significant improvement in locomotor function and enhanced growth of cholera toxin B subunit-positive sensory axons and sprouting of serotonergic fibers Proteus vulgaris
4.2.2.21 medicine sustained delivery of thermostabilized chABC enhances axonal sprouting and functional recovery after spinal cord injury. Animals treated with thermostabilized chABC in combination with sustained neurotrophin-3 delivery show significant improvement in locomotor function and enhanced growth of cholera toxin B subunit-positive sensory axons and sprouting of serotonergic fibers Proteus vulgaris

General Stability

EC Number General Stability Organism
4.2.2.21 microtube-encapsulated chABC is biologically active for 2 weeks Proteus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.21 97000 116000 chABC alone migrates between 97 kDa and 116 kDa, SDS-PAGE Proteus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.20 Proteus vulgaris
-
-
-
4.2.2.21 Proteus vulgaris
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.20 commercial preparation
-
 Proteus vulgaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.21 chondroitin sulfate proteoglycan ChABC digests the glycosaminoglycan side chain of chondroitin sulfate proteoglycan Proteus vulgaris ?
-
 ?
4.2.2.21 decorin
-
 Proteus vulgaris ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.21 chABC
-
 Proteus vulgaris
4.2.2.21 chondroitinase ABC
-
 Proteus vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.21 37
-
 chABC loses ist enzymatic activity rapidly at 37°C, while thermostabilized chABC remains active at 37°C in vitro for up to 4 weeks Proteus vulgaris