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Literature summary extracted from

  • Dreyfus, C.; Lemaire, D.; Mari, S.; Pignol, D.; Arnoux, P.
    Crystallographic snapshots of iterative substrate translocations during nicotianamine synthesis in Archaea (2009), Proc. Natl. Acad. Sci. USA, 106, 16180-16184.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.43 overexpressed as a C-terminal His tag protein in Escherichia coli BL21 (DE3) Methanothermobacter thermautotrophicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.43 at 20°C using the hanging drop vapor diffusion method Methanothermobacter thermautotrophicus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.43 E81Q mutant binds the substrate but not the product Methanothermobacter thermautotrophicus
2.5.1.43 E81Q/Y107F inactive mutant Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.43 S-adenosyl-L-methionine Methanothermobacter thermautotrophicus
-
 5'-S-methyl-5'-thioadenosine + nicotianamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.43 Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.43
-
 Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.43 S-adenosyl-L-methionine
-
 Methanothermobacter thermautotrophicus 5'-S-methyl-5'-thioadenosine + nicotianamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.43 MtNAS
-
 Methanothermobacter thermautotrophicus
2.5.1.43 NAS
-
 Methanothermobacter thermautotrophicus
2.5.1.43 nicotianamine synthase
-
 Methanothermobacter thermautotrophicus