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Literature summary extracted from
- Attachment and entry of Chlamydia have distinct requirements for host protein disulfide isomerase (2009), PLoS Pathog., 5, e1000357.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.4.1 | expression of enzymatically nonfunctional PDI can restore Chlamydia sp. attachment but not entry into mutant CHO6 cells, while expression of functional PDI restores the complete Chlamydia infection, overview | Cricetulus griseus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.4.1 | additional information | a naturally occuring mutant strain with mutated, nonfunctional PDI shows complete resistance to infection, attachement and entry, of Chlamydia species, expression of enzymatically nonfunctional PDI can restore Chlamydia sp. attachment but not entry into mutant CHO6 cells, while expression of functional PDI restores the complete Chlamydia infection, overview | Cricetulus griseus |
| 5.3.4.1 | additional information | HeLa cells are transfected with PDI-targeting siRNA for PDI knockdown leading to resistance to Chlamydia inefctions | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.4.1 | bacitracin | a PDI-specific inhibitor | Cricetulus griseus |  |
| 5.3.4.1 | bacitracin | a PDI-specific inhibitor | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.3.4.1 | cell surface | - |
Cricetulus griseus | 9986 | - |
| 5.3.4.1 | cell surface | - |
Homo sapiens | 9986 | - |
| 5.3.4.1 | membrane | - |
Cricetulus griseus | 16020 | - |
| 5.3.4.1 | membrane | - |
Homo sapiens | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.4.1 | additional information | Cricetulus griseus | the PDI protein is necessary for Chlamydia attachment, but the bacteria apparently do not bind directly to cell-associated PDI, suggesting that Chlamydia attaches to a host protein(s) associated with PDI. PDI enzymatic activity is necessary for bacterial entry but not for attachment, cell surface PDI-mediated reduction triggers Chlamydia entry into cells, molecular mechanism, overview | ? | - |
? | |
| 5.3.4.1 | additional information | Homo sapiens | the PDI protein is necessary for Chlamydia attachment, but the bacteria apparently do not bind directly to cell-associated PDI, suggesting that Chlamydia attaches to a host protein(s) associated with PDI. PDI enzymatic activity is necessary for bacterial entry but not for attachment, cell surface PDI-mediated reduction triggers Chlamydia entry into cells, molecular mechanism, overview | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.4.1 | Cricetulus griseus | - |
- |
- |
| 5.3.4.1 | Homo sapiens | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.3.4.1 | CHO-K1 cell | - |
Cricetulus griseus | - |
| 5.3.4.1 | HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.4.1 | additional information | the PDI protein is necessary for Chlamydia attachment, but the bacteria apparently do not bind directly to cell-associated PDI, suggesting that Chlamydia attaches to a host protein(s) associated with PDI. PDI enzymatic activity is necessary for bacterial entry but not for attachment, cell surface PDI-mediated reduction triggers Chlamydia entry into cells, molecular mechanism, overview | Cricetulus griseus | ? | - |
? | |
| 5.3.4.1 | additional information | the PDI protein is necessary for Chlamydia attachment, but the bacteria apparently do not bind directly to cell-associated PDI, suggesting that Chlamydia attaches to a host protein(s) associated with PDI. PDI enzymatic activity is necessary for bacterial entry but not for attachment, cell surface PDI-mediated reduction triggers Chlamydia entry into cells, molecular mechanism, overview | Homo sapiens | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.4.1 | PDI | - |
Cricetulus griseus |
| 5.3.4.1 | PDI | - |
Homo sapiens |
| 5.3.4.1 | protein disulfide isomerase | - |
Cricetulus griseus |
| 5.3.4.1 | protein disulfide isomerase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.4.1 | 37 | - |
assay at | Cricetulus griseus |
| 5.3.4.1 | 37 | - |
assay at | Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.3.4.1 | physiological function | the enzyme is required for infection by Chlamydia species | Homo sapiens |
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