BRENDA - Enzyme Database

Accumulation of hydroxycinnamic acid amides induced by pathogen infection and identification of agmatine coumaroyltransferase in Arabidopsis thaliana

Muroi, A.; Ishihara, A.; Tanaka, C.; Ishizuka, A.; Takabayashi, J.; Miyoshi, H.; Nishioka, T.; Planta 230, 517-527 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.64
PCR-amplification, expression in Escherichia coli BL21(DE3)
Arabidopsis thaliana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.64
agmatine
the presence of this substrate inhibits the enzyme reaction with putrescine
Arabidopsis thaliana
2.3.1.64
enterokinase
the removal of the thioredoxin domain by enterokinase decreases the enzyme activity but does not alter the relative amounts of products
Arabidopsis thaliana
2.3.1.64
additional information
putrescine or feruloyl-CoA do not inhibit the enzyme reaction with agmantine and p-coumaroyl-CoA
Arabidopsis thaliana
2.3.1.64
p-Coumaroyl-CoA
the presence of this substrate inhibits the enzyme reaction with feruloyl-CoA
Arabidopsis thaliana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.64
70000
-
SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.64
feruloyl-CoA + agmatine
Arabidopsis thaliana
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
CoA + feruloyl agmatine
-
-
?
2.3.1.64
feruloyl-CoA + putrescine
Arabidopsis thaliana
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
CoA + feruloylputrescine
-
-
?
2.3.1.64
p-coumaroyl-CoA + agmatine
Arabidopsis thaliana
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
CoA + p-coumaroylagmatine
-
-
?
2.3.1.64
p-coumaroyl-CoA + putrescine
Arabidopsis thaliana
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
CoA + p-coumaroylputrescine
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.64
Arabidopsis thaliana
-
wild-type and mutants, infection of rosette leaves with the fungus Alternaria brassicicola strain MAFF237450
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.64
frozen leaves are homogenized in 100 mM Tris buffer, pH 7.5, containing 10 mM 2-mercaptoethanol and 5 mM DTT, centrifugation, supernatant applied to Sephadex G-50 column, fluent used for enzyme assays, or recombinant Escherichia coli cells are harvested and resuspended in 100 mM Tris buffer, pH 7.5, containing 1 mM EDTA, 50 mM NaCl, 10% glycerol, lysed with lysozyme, sonicated, centrifuged, supernatant used for enzyme assays and SDS-PAGE
Arabidopsis thaliana
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.64
leaf
cut off rosette leaves infected with the fungus Alternaria brassicicola
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.64
0.00000003
-
with substrates p-coumaroyl-CoA + agmatine + putrescine 0.0018 nmol coumaroylputrescine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.000000085
-
with substrates feruloyl-CoA + putrescine 0.0051 nmol feruloylputrescine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.000000933
-
with substrates p-coumaroyl-CoA + putrescine 0.056 nmol p-coumaroylputrescine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.00002
-
with substrates p-coumaroyl-CoA + feruloyl-CoA + agmatine 1.2 nmol feruloylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.00004
-
with substrates feruloyl-CoA + agmatine 2.4 nmol feruloylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.000112
-
with substrates p-coumaroyl-CoA + agmatine 6.7 nmol p-coumaroylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.000123
-
with substrates p-coumaroyl-CoA + agmatine + putrescine 7.4 nmol p-coumaroylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.000187
-
with substrates p-coumaroyl-CoA + feruloyl-CoA + agmatine 11.2 nmol p-coumaroylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.64
feruloyl-CoA + agmatine
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
706386
Arabidopsis thaliana
CoA + feruloyl agmatine
-
-
-
?
2.3.1.64
feruloyl-CoA + putrescine
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
706386
Arabidopsis thaliana
CoA + feruloylputrescine
-
-
-
?
2.3.1.64
p-coumaroyl-CoA + agmatine
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
706386
Arabidopsis thaliana
CoA + p-coumaroylagmatine
-
-
-
?
2.3.1.64
p-coumaroyl-CoA + putrescine
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
706386
Arabidopsis thaliana
CoA + p-coumaroylputrescine
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.64
PCR-amplification, expression in Escherichia coli BL21(DE3)
Arabidopsis thaliana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.64
agmatine
the presence of this substrate inhibits the enzyme reaction with putrescine
Arabidopsis thaliana
2.3.1.64
enterokinase
the removal of the thioredoxin domain by enterokinase decreases the enzyme activity but does not alter the relative amounts of products
Arabidopsis thaliana
2.3.1.64
additional information
putrescine or feruloyl-CoA do not inhibit the enzyme reaction with agmantine and p-coumaroyl-CoA
Arabidopsis thaliana
2.3.1.64
p-Coumaroyl-CoA
the presence of this substrate inhibits the enzyme reaction with feruloyl-CoA
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.64
70000
-
SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.64
feruloyl-CoA + agmatine
Arabidopsis thaliana
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
CoA + feruloyl agmatine
-
-
?
2.3.1.64
feruloyl-CoA + putrescine
Arabidopsis thaliana
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
CoA + feruloylputrescine
-
-
?
2.3.1.64
p-coumaroyl-CoA + agmatine
Arabidopsis thaliana
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
CoA + p-coumaroylagmatine
-
-
?
2.3.1.64
p-coumaroyl-CoA + putrescine
Arabidopsis thaliana
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
CoA + p-coumaroylputrescine
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.64
frozen leaves are homogenized in 100 mM Tris buffer, pH 7.5, containing 10 mM 2-mercaptoethanol and 5 mM DTT, centrifugation, supernatant applied to Sephadex G-50 column, fluent used for enzyme assays, or recombinant Escherichia coli cells are harvested and resuspended in 100 mM Tris buffer, pH 7.5, containing 1 mM EDTA, 50 mM NaCl, 10% glycerol, lysed with lysozyme, sonicated, centrifuged, supernatant used for enzyme assays and SDS-PAGE
Arabidopsis thaliana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.64
leaf
cut off rosette leaves infected with the fungus Alternaria brassicicola
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.64
0.00000003
-
with substrates p-coumaroyl-CoA + agmatine + putrescine 0.0018 nmol coumaroylputrescine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.000000085
-
with substrates feruloyl-CoA + putrescine 0.0051 nmol feruloylputrescine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.000000933
-
with substrates p-coumaroyl-CoA + putrescine 0.056 nmol p-coumaroylputrescine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.00002
-
with substrates p-coumaroyl-CoA + feruloyl-CoA + agmatine 1.2 nmol feruloylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.00004
-
with substrates feruloyl-CoA + agmatine 2.4 nmol feruloylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.000112
-
with substrates p-coumaroyl-CoA + agmatine 6.7 nmol p-coumaroylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.000123
-
with substrates p-coumaroyl-CoA + agmatine + putrescine 7.4 nmol p-coumaroylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
2.3.1.64
0.000187
-
with substrates p-coumaroyl-CoA + feruloyl-CoA + agmatine 11.2 nmol p-coumaroylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.64
feruloyl-CoA + agmatine
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
706386
Arabidopsis thaliana
CoA + feruloyl agmatine
-
-
-
?
2.3.1.64
feruloyl-CoA + putrescine
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
706386
Arabidopsis thaliana
CoA + feruloylputrescine
-
-
-
?
2.3.1.64
p-coumaroyl-CoA + agmatine
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
706386
Arabidopsis thaliana
CoA + p-coumaroylagmatine
-
-
-
?
2.3.1.64
p-coumaroyl-CoA + putrescine
30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors
706386
Arabidopsis thaliana
CoA + p-coumaroylputrescine
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
2.3.1.64
Arabidopsis thaliana
upon infection with Alternaria brassicicola enzyme activity in leaves increases markedly from near zero to almost 20 nmol/mg protein/h within 24 h coinciding with an increase in gene transcripts and in the synthesis products, that accumulate in the area of the lesion, slow decrease beyond 24 h
up
General Information
EC Number
General Information
Commentary
Organism
2.3.1.64
metabolism
last step in the synthesis of hydroxycinnamic acid amides in plants (anti-pathogenic metabolites), in Arabidopsis thaliana infected with Alternaria brassicicola p-coumaroylagmatine, feruloylagmatine, p-coumaroylputrescine, and feruloylputrescine are identified as anti-fungal agents in vitro and in vivo
Arabidopsis thaliana
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.3.1.64
metabolism
last step in the synthesis of hydroxycinnamic acid amides in plants (anti-pathogenic metabolites), in Arabidopsis thaliana infected with Alternaria brassicicola p-coumaroylagmatine, feruloylagmatine, p-coumaroylputrescine, and feruloylputrescine are identified as anti-fungal agents in vitro and in vivo
Arabidopsis thaliana
Expression (protein specific)
EC Number
Organism
Commentary
Expression
2.3.1.64
Arabidopsis thaliana
upon infection with Alternaria brassicicola enzyme activity in leaves increases markedly from near zero to almost 20 nmol/mg protein/h within 24 h coinciding with an increase in gene transcripts and in the synthesis products, that accumulate in the area of the lesion, slow decrease beyond 24 h
up