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Literature summary extracted from

  • Nitsch, L.M.; Oplaat, C.; Feron, R.; Ma, Q.; Wolters-Arts, M.; Hedden, P.; Mariani, C.; Vriezen, W.H.
    Abscisic acid levels in tomato ovaries are regulated by LeNCED1 and SlCYP707A1 (2009), Planta, 229, 1335-1346.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.137 4-chloroindole-3-acetic acid auxin, stimulates Solanum lycopersicum
1.14.14.137 abscisic acid stimulates Solanum lycopersicum
1.14.14.137 additional information CYP707A1 is not affected by gibberellin 3 treatment, in contrast to pollination, which has a stimulating effect Solanum lycopersicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.137 coding region (base 6-1,478) amplified and cloned in the Gateway entry vector pENTR/D-TOPO. CYP707A1 coding region recombined between the Cauliflower Mosaic Virus 35S promoter in the pGD625 vector and the NOPALINE SYNTHASE terminator. Transgenic plants generated by Agrobacterium tumefaciens-mediated transformation Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.137 Solanum lycopersicum A9QNE7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.137 fruit
-
 Solanum lycopersicum
-
1.14.14.137 additional information high level in plant placenta Solanum lycopersicum
-
1.14.14.137 pericarp low level Solanum lycopersicum
-
1.14.14.137 plant ovary
-
 Solanum lycopersicum
-
1.14.14.137 plant ovule high level Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.137 (+)-abscisic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Solanum lycopersicum 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.137 (+)ABA 8'-hydroxylase
-
 Solanum lycopersicum
1.14.14.137 CYP707A1
-
 Solanum lycopersicum

Expression

EC Number Organism Comment Expression
1.14.14.137 Solanum lycopersicum mRNA concentration of CYP707A1 strongly increases after pollination in whole ovaries. CYP707A1 mRNA levels are several 100fold upregulated after pollination, specifically in ovules/placenta and not in pericarp. CYP707A1 overexpression line has approximately 45fold higher mRNA levels than wild-type up

General Information

EC Number General Information Comment Organism
1.14.14.137 physiological function CYP707A1 is most important for abscisic acid catabolism in pollinated ovaries. Transgenic plants, overexpressing CYP707A1, have reduced abscisic acid levels and exhibit abscisic acid-deficient phenotypes. Initiation of adventitious root growth on the stem of the CYP707A1 overexpression plants Solanum lycopersicum