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Literature summary extracted from

  • Meissner, D.; Albert, A.; Boettcher, C.; Strack, D.; Milkowski, C.
    The role of UDP-glucose:hydroxycinnamate glucosyltransferases in phenylpropanoid metabolism and the response to UV-B radiation in Arabidopsis thaliana (2008), Planta, 228, 663-674.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.120 binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred Arabidopsis thaliana
2.4.1.126 binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.120 UDP-alpha-D-glucose + sinapate Arabidopsis thaliana
-
 UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?
2.4.1.126 UDP-alpha-D-glucose + 4-hydroxycinnamate Arabidopsis thaliana
-
 UDP + 4-O-(beta-D-glucopyranosyl)-4-hydroxycinnamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.120 Arabidopsis thaliana Q9LVF0
-
-
2.4.1.126 Arabidopsis thaliana O23402
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.120 leaf low activity in mature leaves Arabidopsis thaliana
-
2.4.1.120 seed
-
 Arabidopsis thaliana
-
2.4.1.120 seedling
-
 Arabidopsis thaliana
-
2.4.1.126 leaf low activity in mature leaves Arabidopsis thaliana
-
2.4.1.126 seed
-
 Arabidopsis thaliana
-
2.4.1.126 seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.120 UDP-alpha-D-glucose + sinapate
-
 Arabidopsis thaliana UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?
2.4.1.126 UDP-alpha-D-glucose + 4-hydroxycinnamate
-
 Arabidopsis thaliana UDP + 4-O-(beta-D-glucopyranosyl)-4-hydroxycinnamate
-
 ?
2.4.1.126 UDP-alpha-D-glucose + sinapate
-
 Arabidopsis thaliana UDP + 4-O-(beta-D-glucopyranosyl)-4-hydroxycinnamate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.120 30
-
 assay at Arabidopsis thaliana
2.4.1.126 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.120 6
-
 assay at Arabidopsis thaliana
2.4.1.126 6
-
 assay at Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
2.4.1.126 Arabidopsis thaliana given the impact of UGT84A3 overexpression on the amount of cell wall-associated hydroxycinnamates, analysis of the cell wall fraction of the insertion knock-out mutant UGT84A3 shows a significant decrease of the 4-coumarate content in cell walls of null mutant whereas the levels of ferulate and sinapate are not changed compared to the wild type additional information
2.4.1.126 Arabidopsis thaliana exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and leads to a transient increase in sinapoylglucose and sinapoylmalate concentrations, compared to the wild type, crude protein extracts from leaves of UGT84A1 overexpression plants displayed increased activity toward 4-coumarate and ferulate, the preferred in vitro substrates, the overexpressing line UGT84A1 exhibits increased levels of sinapoylglucose in seeds and seedlings, the seed sinapine content is slightly increased in UGT84A1, overexpression of UGT84A1 results in a significant increase of cell wall-bound sinapate up
2.4.1.126 Arabidopsis thaliana exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and leads to a transient increase in sinapoylglucose and sinapoylmalate concentrations, leaves of UGT84A3 overexpression displayed a significantly higher capacity to synthesize feruloylglucose, the overexpressing line UGT84A3 exhibits increased levels of sinapoylglucose in seeds and seedlings, the seed sinapine content is slightly increased in UGT84A3, analysis of liberated from cell walls reveals for UGT84A3 a significant increase in cell wall-associated 4-coumarate, ferulate and sinapate up
2.4.1.126 Arabidopsis thaliana exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and leads to a transient increase in sinapoylglucose and sinapoylmalate concentrations, overexpression plants did not display changes in UGT specificity of leave extracts, the overexpressing line UGT84A4 exhibits increased levels of sinapoylglucose in seeds and seedlings, the seed sinapine content is slightly increased up
2.4.1.126 Arabidopsis thaliana exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and leads to a transient increase in sinapoylglucose and sinapoylmalate concentrations, UGT84A2 overexpression caused increasing activity toward sinapate, the overexpressing line UGT84A2 exhibits increased levels of sinapoylglucose in seeds and seedlings, the seed sinapine content is slightly increased in UGT84A2, mature leaves of UGT84A2 show a slight increase in the sinapoylmalate content, overexpression of UGT84A2 results in a significant increase of cell wall-bound sinapate up

General Information

EC Number General Information Comment Organism
2.4.1.126 metabolism phenylpropanoid metabolism Arabidopsis thaliana
2.4.1.126 physiological function specific role for UGT84A3 in the accumulation of cell wall-bound 4-coumarate Arabidopsis thaliana