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Literature summary extracted from

  • Whitney, S.M.; Kane, H.J.; Houtz, R.L.; Sharwood, R.E.
    Rubisco oligomers composed of linked small and large subunits assemble in tobacco plastids and have higher affinities for CO2 and O2 (2009), Plant Physiol., 149, 1887-1895.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.39 expressed in Escherichia coli Nicotiana tabacum

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.39 additional information the S and L subunits of Rubisco are linked together using a flexible 40-amino acid tether. By replacing the rbcL in tobacco plastids with an artificial gene coding for a S40L fusion peptide, the fusions readily assemble into catalytic (S40L)8 and (S40L)16 oligomers that are devoid of unlinked S subunits. While there is little or no change in CO2/O2 specificity or carboxylation rate of the Rubisco oligomers, their Kms for CO2 and O2 are reduced 10% to 20% and 45%, respectively. In young maturing leaves of the plastome transformants (ANtS40L), the S40L-Rubisco levels are approximately 20% that of wild-type controls despite turnover of the S40L-Rubisco oligomers being only slightly enhanced relative to wild-type Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.39 0.0095
-
 CO2 ANtS40L Rubisco oligomer complex, using soluble leaf protein extract Nicotiana tabacum
4.1.1.39 0.0102
-
 CO2 rubisco oligomer (S40L)16 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification Nicotiana tabacum
4.1.1.39 0.0106
-
 CO2 rubisco oligomer (S40L)8 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification Nicotiana tabacum
4.1.1.39 0.0118
-
 CO2 wild-type, measured using fractions 12 and 13 following Superdex 200 HR purification Nicotiana tabacum
4.1.1.39 0.0122
-
 CO2 wild-type, using soluble leaf protein extract Nicotiana tabacum
4.1.1.39 0.112
-
 O2 ANtS40L Rubisco oligomer complex, using soluble leaf protein extract Nicotiana tabacum
4.1.1.39 0.203
-
 CO2 wild-type, using soluble leaf protein extract Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Nicotiana tabacum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.39 using Q-sepharose anion exchange chromatography and gel filtration Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 Nicotiana tabacum 2 3-phospho-D-glycerate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.39 hexadecamer wild-type enzyme is an assembly of eight plastid-encoded large (L) and eight nucleus-encoded small (S) subunits froming a hexadecameric (L8S8) enzyme Nicotiana tabacum

Synonyms

EC Number Synonyms Comment Organism
4.1.1.39 Ribulose 1,5-bisphosphate carboxylase/oxygenase
-
 Nicotiana tabacum
4.1.1.39 Rubisco
-
 Nicotiana tabacum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.39 0.42
-
 O2 ANtS40L Rubisco oligomer complex, using soluble leaf protein extract Nicotiana tabacum
4.1.1.39 0.65
-
 CO2 wild-type, using soluble leaf protein extract Nicotiana tabacum
4.1.1.39 2.9
-
 CO2 ANtS40L Rubisco oligomer complex, using soluble leaf protein extract Nicotiana tabacum
4.1.1.39 2.9
-
 CO2 rubisco oligomer (S40L)8 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification Nicotiana tabacum
4.1.1.39 3.1
-
 CO2 rubisco oligomer (S40L)16 composed of linked small and large subunits, measured using fractions 7 and 8 following Superdex 200 HR purification Nicotiana tabacum
4.1.1.39 3.1
-
 CO2 wild-type, using soluble leaf protein extract Nicotiana tabacum
4.1.1.39 3.3
-
 CO2 wild-type, measured using fractions 12 and 13 following Superdex 200 HR purification Nicotiana tabacum