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Literature summary extracted from

  • Zhou, J.M.; Seo, Y.W.; Ibrahim, R.K.
    Biochemical characterization of a putative wheat caffeic acid O-methyltransferase (2008), Plant Physiol. Biochem., 47, 322-326.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.169 expression in Escherichia coli Triticum aestivum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.169 additional information no requirement for Mg2+ Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.169 38900
-
 x * 38900, calculated from sequence, SDS-PAGE Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.169 Triticum aestivum Q84N28
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.169
-
 Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
2.1.1.169 4°C, almost no loss of catalytic activity when stored for 3-4 weeks Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.169 3 S-adenosyl-L-methionine + tricetin TaOMT1 (TaCOMT1) catalyzes the sequential methylation of tricetin to its 3'-O-monomethyl-derivative (selgin), 3',5'-O-dimethyl-derivative (tricin) and 3',4',5'-O-trimethyl derivative. 3',5'-O-dimethyltricetin appears to be the major reaction product. The enzyme also catalyzes methylation of luteolin, quercetin and eriodictyol Triticum aestivum 3 S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
-
 ?
2.1.1.169 S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin
-
 Triticum aestivum S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
-
 ?
2.1.1.169 S-adenosyl-L-methionine + 3'-O-methyltricetin
-
 Triticum aestivum S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
-
 ?
2.1.1.169 S-adenosyl-L-methionine + tricetin
-
 Triticum aestivum S-adenosyl-L-homocysteine + 3'-O-methyltricetin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.169 ? x * 38900, calculated from sequence, SDS-PAGE Triticum aestivum

Synonyms

EC Number Synonyms Comment Organism
2.1.1.169 TaCOMT1
-
 Triticum aestivum
2.1.1.169 TaOMT
-
 Triticum aestivum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.169 Triticum aestivum calculated from sequence
-
 5.75