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Literature summary extracted from

  • Zhang, C.; Meng, Q.; Zhang, M.; Huang, F.; Gai, J.; Yu, D.
    Characterization of O-acetylserine(thiol)lyase-encoding genes reveals their distinct but cooperative expression in cysteine synthesis of soybean [Glycine max (L.) Merr.] (2008), Plant Mol. Biol. Rep., 26, 277-291.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.47 expressed in Escherichia coli as GST-fusion proteins Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.062
-
 Sulfide
-
 Glycine max
2.5.1.47 0.063
-
 O-acetyl-L-serine
-
 Glycine max
2.5.1.47 0.105
-
 O-acetyl-L-serine
-
 Glycine max
2.5.1.47 0.11
-
 Sulfide
-
 Glycine max
2.5.1.47 0.116
-
 O-acetyl-L-serine
-
 Glycine max
2.5.1.47 0.355
-
 O-acetyl-L-serine
-
 Glycine max
2.5.1.47 0.53
-
 Sulfide
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 58000
-
 calculated from cDNA Glycine max
2.5.1.47 60000
-
 calculated from cDNA Glycine max
2.5.1.47 66000
-
 calculated from cDNA Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Glycine max A3RM03
-
-
2.5.1.47 Glycine max A3RM04
-
-
2.5.1.47 Glycine max A3RM05
-
-
2.5.1.47 Glycine max A3RM06
-
-
2.5.1.47 Glycine max A5YT86
-
-
2.5.1.47 Glycine max A5YT88
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47 using gel filtration Glycine max
2.5.1.47 using Glutathione Sepharose 4B column Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 flower
-
 Glycine max
-
2.5.1.47 leaf
-
 Glycine max
-
2.5.1.47 root roots of seedlings Glycine max
-
2.5.1.47 seed
-
 Glycine max
-
2.5.1.47 seed mainly expressed in developing seeds Glycine max
-
2.5.1.47 stem young stems Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 0.262
-
-
 Glycine max
2.5.1.47 0.419
-
-
 Glycine max
2.5.1.47 0.489
-
-
 Glycine max
2.5.1.47 0.724
-
-
 Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-serine + sulfide
-
 Glycine max L-Cys + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 GmOAS-TL1
-
 Glycine max
2.5.1.47 GmOAS-TL2
-
 Glycine max
2.5.1.47 GmOAS-TL3
-
 Glycine max
2.5.1.47 GmOAS-TL4
-
 Glycine max
2.5.1.47 GmOAS-TL6
-
 Glycine max
2.5.1.47 GmOAS-TL7
-
 Glycine max

General Information

EC Number General Information Comment Organism
2.5.1.47 physiological function cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL1 grow in the M9 minimal medium in the absence of cysteine Glycine max
2.5.1.47 physiological function cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL2 does not grow in the M9 minimal medium in the absence of cysteine Glycine max
2.5.1.47 physiological function cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL3 grow in the M9 minimal medium in the absence of cysteine Glycine max
2.5.1.47 physiological function cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL4 grow in the M9 minimal medium in the absence of cysteine Glycine max
2.5.1.47 physiological function cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL6 grow in the M9 minimal medium in the absence of cysteine Glycine max
2.5.1.47 physiological function cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL7 does not grow in the M9 minimal medium in the absence of cysteine Glycine max