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Literature summary extracted from

  • Meng, F.; Hu, L.; Wang, S.; Sui, X.; Wei, L.; Wei, Y.; Sun, J.; Zhang, Z.
    Effects of exogenous abscisic acid (ABA) on cucumber seedling leaf carbohydrate metabolism under low temperature (2008), Plant Growth Regul., 56, 233-244.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.67 abscisic acid in concentration of 50 and 150 micromol/l Cucumis sativus
2.4.1.67 additional information higher enzyme activity in the cold-sensitive cultivar, Guonong No. 41 Cucumis sativus
2.4.1.67 additional information increase of activity for 3 days in the cold-tolerant cultivar, Guonong No. 25 Cucumis sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.67 abscisic acid in concentration of 250 micromol/l Cucumis sativus
2.4.1.67 additional information decrease of activity in the cold-sensitive culitvar, Guonong No. 41 Cucumis sativus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.67 Cucumis sativus
-
 a cold-tolerant cultivar, Guonong No. 25, and a cold sensitive cultivar, Guonong No. 41
-
3.2.1.22 Cucumis sativus
-
 a cold-tolerant cultivar, Guonong No. 25, and a cold sensitive cultivar, Guonong No. 41
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.67 desalted Cucumis sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.67 leaf
-
 Cucumis sativus
-
3.2.1.22 leaf
-
 Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.67 0.0004167
-
 value about, after 6 days of cold treatment and 250 micromol/l exogenous abscisic acid Cucumis sativus
2.4.1.67 0.00167
-
 value about, cold-sensitve cultivar, Guonong No. 41, grown under low temperature for 9 days Cucumis sativus
2.4.1.67 0.00167
-
 value about, cold-tolerant cultivar, Guonong No. 25, grown under normal temperature Cucumis sativus
2.4.1.67 0.0033
-
 value about, cold-sensitve cultivar, Guonong No. 41, grown under low temperature for 3 days Cucumis sativus
2.4.1.67 0.0033
-
 value about, cold-tolerant cultivar, Guonong No. 25, grown under low temperature for 3 days Cucumis sativus
2.4.1.67 0.004167
-
 value about, after 6 days of cold treatment and 50 micromol/l exogenous abscisic acid Cucumis sativus
2.4.1.67 0.00458
-
 value about, after 6 days of cold treatment and 150 micromol/l exogenous abscisic acid Cucumis sativus
2.4.1.67 0.0067
-
 value about, cold-sensitve cultivar, Guonong No. 41, grown under normal temperature Cucumis sativus
3.2.1.22 0.000833
-
 value about, grown under low temperature for at least 3 days Cucumis sativus
3.2.1.22 0.00167
-
 value about, grown under normal temperature for at least 3 days Cucumis sativus
3.2.1.22 0.00375
-
 value about, cold-sensitve cultivar, Guonong No. 41 Cucumis sativus
3.2.1.22 0.004167
-
 value about, cold-tolerant cultivar, Guonong No. 25 Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.67 galactinol + raffinose assay at pH 7.0, 25°C, 90 min, reaction terminated by addition of NaOH Cucumis sativus ?
-
 ?
3.2.1.22 raffinose + H2O assay at pH 7.5, 37°C, 20 min, reaction terminated by keeping the reaction mixture in boiling water for 2 min Cucumis sativus D-galactose + sucrose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.67 stachyose synthase
-
 Cucumis sativus
2.4.1.67 STS
-
 Cucumis sativus
3.2.1.22 alkaline alpha-galactosidase
-
 Cucumis sativus
3.2.1.22 alpha-Gal
-
 Cucumis sativus
3.2.1.22 alpha-galactosidase
-
 Cucumis sativus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.67 25
-
 assay at Cucumis sativus
3.2.1.22 37
-
 assay at Cucumis sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.67 7
-
 assay at Cucumis sativus
3.2.1.22 7.5
-
 assay at Cucumis sativus

General Information

EC Number General Information Comment Organism
2.4.1.67 metabolism stachyose biosynthesis Cucumis sativus
3.2.1.22 metabolism stachyose biosynthesis Cucumis sativus