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Literature summary extracted from

  • Madsen, C.T.; Mengel-Jorgensen, J.; Kirpekar, F.; Douthwaite, S.
    Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry (2003), Nucleic Acids Res., 31, 4738-4746.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.189 expression of a intein-fusion protein with an N-terminal His-tag Escherichia coli
2.1.1.190 expression of a intein-fusion protein with an C-terminal His-tag Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.189 Escherichia coli P75817
-
-
2.1.1.190 Escherichia coli P55135
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.190
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.189 S-adenosyl-L-methionine + uracil747 in 23S rRNA it is not possible to generate a recombinant version of YbjF that retains in vitro activity, so the function of this enzyme is defined in vivo by engineering a ybjF knockout strain Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?
2.1.1.190 S-adenosyl-L-methionine + uracil1939 in 23S rRNA a purified recombinant version of YgcA retains its activity and specificity, and methylates U1939 in an RNA transcript in vitro Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.189 RNA uridine methyltransferase B
-
 Escherichia coli
2.1.1.189 RumB
-
 Escherichia coli
2.1.1.189 YbjF
-
 Escherichia coli
2.1.1.190 RNA uridine methyltransferase A
-
 Escherichia coli
2.1.1.190 RumA
-
 Escherichia coli
2.1.1.190 YgcA
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.1.1.189 malfunction comparison of the methylation patterns in 23S rRNAs from YbjF+ and YbjF- strains shows that the latter differs only in the lack of the 5-methyluracil747 modification Escherichia coli