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Literature summary extracted from
- Purification and characterization of a thermostable MnSOD from the thermophilic fungus Chaetomium thermophilum (2008), Mycologia, 100, 375-380.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.15.1.1 | additional information | no inhibition by KCN and H2O2 | Thermochaetoides thermophila | |
| 1.15.1.1 | NaN3 | - |
Thermochaetoides thermophila |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.15.1.1 | Mn2+ | 0.00205 mg/mg of protein | Thermochaetoides thermophila | |
| 1.15.1.1 | additional information | the purified enzyme contains no Fe | Thermochaetoides thermophila |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 23500 | - |
4 * 23500, SDS-PAGE | Thermochaetoides thermophila |
| 1.15.1.1 | 94400 | - |
gel filtration | Thermochaetoides thermophila |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.15.1.1 | Thermochaetoides thermophila | - |
- |
- |
| 1.15.1.1 | Thermochaetoides thermophila CT2 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.15.1.1 | native enzyme 7.49fold to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and hydrophobic interaction chromatography | Thermochaetoides thermophila |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 115.8 | - |
purified native enzyme | Thermochaetoides thermophila |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.15.1.1 | More | the enzyme contains the sequence TLPDLKYD at the N-terminus | Thermochaetoides thermophila |
| 1.15.1.1 | tetramer | 4 * 23500, SDS-PAGE | Thermochaetoides thermophila |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.15.1.1 | MnSOD | - |
Thermochaetoides thermophila |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 60 | - |
- |
Thermochaetoides thermophila |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 35 | 90 | - |
Thermochaetoides thermophila |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 50 | 60 | purified enzyme, completely stable | Thermochaetoides thermophila |
| 1.15.1.1 | 70 | - |
60 min, 60% activity remaining | Thermochaetoides thermophila |
| 1.15.1.1 | 80 | - |
half-life of the purified enzyme is about 25 min | Thermochaetoides thermophila |
| 1.15.1.1 | 90 | - |
purified enzyme, 30 min, 20% activity remaining | Thermochaetoides thermophila |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 7.5 | - |
- |
Thermochaetoides thermophila |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 5 | 10 | - |
Thermochaetoides thermophila |
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Release 2023.1 (January 2023)
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