EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.21 | expressed in Escherichia coli Rosetta(DE3) cells as a fusion protein to glutathione transferase | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.21 | H13I | the mutant shows strongly increased Km compared to the wild type enzyme | Human alphaherpesvirus 1 |
2.7.1.21 | H13I/Y127F | the mutant shows increased Km compared to the wild type enzyme | Human alphaherpesvirus 1 |
2.7.1.21 | I59H | the mutant shows imcreased Km compared to the wild type enzyme | Homo sapiens |
2.7.1.21 | I59L | the mutant shows imcreased Km compared to the wild type enzyme | Homo sapiens |
2.7.1.21 | I59V | the mutant shows imcreased Km compared to the wild type enzyme | Homo sapiens |
2.7.1.21 | I59Y | the mutant shows decreased Km compared to the wild type enzyme | Homo sapiens |
2.7.1.21 | P12N | the mutant shows increased Km compared to the wild type enzyme | Human alphaherpesvirus 1 |
2.7.1.21 | P12N/H13I | the mutant shows decreased Km compared to the wild type enzyme | Human alphaherpesvirus 1 |
2.7.1.21 | P12N/H13I/Y127F | the mutant shows strongly increased Km compared to the wild type enzyme | Human alphaherpesvirus 1 |
2.7.1.21 | P12N/Y127F | the mutant shows strongly increased Km compared to the wild type enzyme | Human alphaherpesvirus 1 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.21 | 1-(3,4-dichlorobenzyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Homo sapiens | |
2.7.1.21 | 1-(3,4-dichlorobenzyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 1-(3,4-dichlorophenyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Homo sapiens | |
2.7.1.21 | 1-(3,4-dichlorophenyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 1-benzyl-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Homo sapiens | |
2.7.1.21 | 1-benzyl-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]thiourea | - |
Homo sapiens | |
2.7.1.21 | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]thiourea | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]urea | - |
Homo sapiens | |
2.7.1.21 | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]urea | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Homo sapiens | |
2.7.1.21 | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 1-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]-3-phenylthiourea | - |
Homo sapiens | |
2.7.1.21 | 1-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]-3-phenylthiourea | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 3'-([[(4-chlorophenyl)carbamothioyl]amino]methyl)-3'-deoxythymidine | - |
Homo sapiens | |
2.7.1.21 | 3'-([[(4-chlorophenyl)carbamothioyl]amino]methyl)-3'-deoxythymidine | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 3'-deoxy-3'-([[(3,4-dichlorophenyl)carbamothioyl]amino]methyl)thymidine | - |
Homo sapiens | |
2.7.1.21 | 3'-deoxy-3'-([[(3,4-dichlorophenyl)carbamothioyl]amino]methyl)thymidine | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 3'-deoxy-3'-([[(4-methoxyphenyl)carbamothioyl]amino]methyl)thymidine | - |
Homo sapiens | |
2.7.1.21 | 3'-deoxy-3'-([[(4-methoxyphenyl)carbamothioyl]amino]methyl)thymidine | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 3'-deoxy-3'-([[(4-methylphenyl)carbamothioyl]amino]methyl)thymidine | - |
Homo sapiens | |
2.7.1.21 | 3'-deoxy-3'-([[(4-methylphenyl)carbamothioyl]amino]methyl)thymidine | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 3'-[([[4-(benzyloxy)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | - |
Homo sapiens | |
2.7.1.21 | 3'-[([[4-(benzyloxy)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 3'-[([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | - |
Homo sapiens | |
2.7.1.21 | 3'-[([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 5'-deoxy-5'-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamothioyl)amino]thymidine | - |
Homo sapiens | |
2.7.1.21 | 5'-deoxy-5'-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamothioyl)amino]thymidine | - |
Human alphaherpesvirus 1 | |
2.7.1.21 | 5'-deoxy-5'-[[(diphenylmethyl)carbamothioyl]amino]thymidine | - |
Homo sapiens | |
2.7.1.21 | 5'-deoxy-5'-[[(diphenylmethyl)carbamothioyl]amino]thymidine | - |
Human alphaherpesvirus 1 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.21 | 0.00023 | - |
thymidine | mutant enzyme P12N/H13I, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 | |
2.7.1.21 | 0.00044 | - |
thymidine | wild type enzyme, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 | |
2.7.1.21 | 0.0039 | - |
thymidine | mutant enzyme P12N, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 | |
2.7.1.21 | 0.0039 | - |
thymidine | thymidine kinase-2 mutant enzyme I59Y, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
2.7.1.21 | 0.0043 | - |
thymidine | wild type thymidine kinase-2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
2.7.1.21 | 0.0051 | - |
thymidine | thymidine kinase-2 mutant enzyme I59V, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
2.7.1.21 | 0.0056 | - |
thymidine | thymidine kinase-2 mutant enzyme I59L, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
2.7.1.21 | 0.006 | - |
thymidine | thymidine kinase-2 mutant enzyme I59H, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
2.7.1.21 | 0.037 | - |
thymidine | mutant enzyme H13I/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 | |
2.7.1.21 | 0.263 | - |
thymidine | mutant enzyme P12N/H13I/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 | |
2.7.1.21 | 0.59 | - |
thymidine | mutant enzyme P12N/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 | |
2.7.1.21 | 0.612 | - |
thymidine | mutant enzyme H13I, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C | Human alphaherpesvirus 1 |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.21 | mitochondrion | beta-thymidine kinase-2 | Homo sapiens | 5739 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.21 | Homo sapiens | - |
- |
- |
2.7.1.21 | Human alphaherpesvirus 1 | P03176 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.21 | glutathione-Sepharose column chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.21 | ATP + thymidine | - |
Homo sapiens | ADP + thymidine 5'-phosphate | - |
? | |
2.7.1.21 | ATP + thymidine | - |
Human alphaherpesvirus 1 | ADP + thymidine 5'-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.21 | beta-thymidine kinase-1 | - |
Homo sapiens |
2.7.1.21 | beta-thymidine kinase-2 | - |
Homo sapiens |
2.7.1.21 | dThd kinase-1 | - |
Homo sapiens |
2.7.1.21 | dThd kinase-2 | - |
Homo sapiens |
2.7.1.21 | thymidine kinase-1 | - |
Homo sapiens |
2.7.1.21 | thymidine kinase-2 | - |
Homo sapiens |
2.7.1.21 | TK-1 | - |
Homo sapiens |
2.7.1.21 | TK-2 | - |
Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.21 | ATP | - |
Homo sapiens | |
2.7.1.21 | ATP | - |
Human alphaherpesvirus 1 |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.1.21 | 0.00015 | - |
- |
Homo sapiens | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]thiourea | |
2.7.1.21 | 0.00042 | - |
- |
Homo sapiens | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]urea | |
2.7.1.21 | 0.00043 | - |
- |
Homo sapiens | 3'-[([[4-(benzyloxy)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | |
2.7.1.21 | 0.00047 | - |
- |
Homo sapiens | 3'-[([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | |
2.7.1.21 | 0.00064 | - |
- |
Homo sapiens | 3'-deoxy-3'-([[(3,4-dichlorophenyl)carbamothioyl]amino]methyl)thymidine | |
2.7.1.21 | 0.0012 | - |
- |
Homo sapiens | 3'-([[(4-chlorophenyl)carbamothioyl]amino]methyl)-3'-deoxythymidine | |
2.7.1.21 | 0.0012 | - |
- |
Homo sapiens | 3'-deoxy-3'-([[(4-methylphenyl)carbamothioyl]amino]methyl)thymidine | |
2.7.1.21 | 0.0031 | - |
- |
Homo sapiens | 3'-deoxy-3'-([[(4-methoxyphenyl)carbamothioyl]amino]methyl)thymidine | |
2.7.1.21 | 0.031 | - |
- |
Homo sapiens | 5'-deoxy-5'-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamothioyl)amino]thymidine | |
2.7.1.21 | 0.038 | - |
- |
Homo sapiens | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
2.7.1.21 | 0.041 | - |
- |
Homo sapiens | 1-(3,4-dichlorobenzyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
2.7.1.21 | 0.046 | - |
- |
Human alphaherpesvirus 1 | 3'-[([[4-chloro-3-(trifluoromethyl)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | |
2.7.1.21 | 0.057 | - |
- |
Homo sapiens | 1-(3,4-dichlorophenyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
2.7.1.21 | 0.087 | - |
- |
Human alphaherpesvirus 1 | 3'-deoxy-3'-([[(3,4-dichlorophenyl)carbamothioyl]amino]methyl)thymidine | |
2.7.1.21 | 0.099 | - |
- |
Homo sapiens | 1-benzyl-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
2.7.1.21 | 0.11 | - |
- |
Homo sapiens | 5'-deoxy-5'-[[(diphenylmethyl)carbamothioyl]amino]thymidine | |
2.7.1.21 | 0.195 | - |
- |
Human alphaherpesvirus 1 | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]thiourea | |
2.7.1.21 | 0.232 | - |
- |
Human alphaherpesvirus 1 | 3'-[([[4-(benzyloxy)phenyl]carbamothioyl]amino)methyl]-3'-deoxythymidine | |
2.7.1.21 | 0.335 | - |
- |
Human alphaherpesvirus 1 | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[(2S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-3-yl]urea | |
2.7.1.21 | 0.365 | - |
- |
Human alphaherpesvirus 1 | 1-(3,4-dichlorobenzyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
2.7.1.21 | 0.392 | - |
- |
Human alphaherpesvirus 1 | 5'-deoxy-5'-[(tricyclo[3.3.1.13,7]dec-1-ylcarbamothioyl)amino]thymidine | |
2.7.1.21 | 0.4 | - |
- |
Human alphaherpesvirus 1 | 3'-([[(4-chlorophenyl)carbamothioyl]amino]methyl)-3'-deoxythymidine | |
2.7.1.21 | 0.405 | - |
- |
Homo sapiens | 1-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]-3-phenylthiourea | |
2.7.1.21 | 0.422 | - |
- |
Human alphaherpesvirus 1 | 1-(3,4-dichlorophenyl)-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
2.7.1.21 | 0.444 | - |
- |
Human alphaherpesvirus 1 | 3'-deoxy-3'-([[(4-methylphenyl)carbamothioyl]amino]methyl)thymidine | |
2.7.1.21 | 0.472 | - |
- |
Human alphaherpesvirus 1 | 1-[4-chloro-3-(trifluoromethyl)phenyl]-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea | |
2.7.1.21 | 0.5 | - |
IC50 above 0.5 mM | Human alphaherpesvirus 1 | 3'-deoxy-3'-([[(4-methoxyphenyl)carbamothioyl]amino]methyl)thymidine | |
2.7.1.21 | 0.5 | - |
IC50 above 0.5 mM | Human alphaherpesvirus 1 | 1-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]-3-phenylthiourea | |
2.7.1.21 | 0.5 | - |
IC50 above 0.5 mM | Human alphaherpesvirus 1 | 5'-deoxy-5'-[[(diphenylmethyl)carbamothioyl]amino]thymidine | |
2.7.1.21 | 0.5 | - |
IC50 above 0.5 mM | Human alphaherpesvirus 1 | 1-benzyl-3-[[(2R,3S,5S)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl]thiourea |