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Literature summary extracted from

  • Geremia, R.A.; Roux, M.; Ferreiro, D.U.; Dauphin-Dubois, R.; Lellouch, A.C.; Ielpi, L.
    Expression and biochemical characterisation of recombinant AceA, a bacterial alpha-mannosyltransferase (1990), Mol. Gen. Genet., 261, 933-940.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.252 overproduced in Escherichia coli. The aceA ORF is subcloned into the expression vector pET29 in frame with the S-tag epitope. The recombinant protein is identified, and culture conditions are optimised for production of the soluble protein Komagataeibacter xylinus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.252 additional information no inhibition by cellobiose Komagataeibacter xylinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.252 GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol Komagataeibacter xylinus the enzyme is involved in the biosynthesis of the exopolysaccharide acetan GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.252 Komagataeibacter xylinus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.252 GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol the enzyme is involved in the biosynthesis of the exopolysaccharide acetan Komagataeibacter xylinus GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.252 GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol AceA is not able to use free cellobiose as a substrate, indicating that the pyrophosphate-lipid moiety is needed for enzymatic activity Komagataeibacter xylinus GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol characterisation of the in vitro reaction product ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.252 AceA
-
 Komagataeibacter xylinus

General Information

EC Number General Information Comment Organism
2.4.1.252 physiological function the enzyme is involved in the biosynthesis of the exopolysaccharide acetan Komagataeibacter xylinus