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Literature summary extracted from

  • Rattanachuen, W.; Joensson, M.; Swedberg, G.; Sirawaraporn, W.
    Probing the roles of non-homologous insertions in the N-terminal domain of Plasmodium falciparum hydroxymethylpterin pyrophosphokinase-dihydropteroate synthase (2009), Mol. Biochem. Parasitol., 168, 135-142.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.15 expressed in Escherichia coli Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.15 DELTA247-306 deletion of the parasite-specific insertion: Km values for (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate and p-aminobenzoate are increased compared to mutant DELTA257-306 but still decreased compared to wild type, catalytic efficacy (kcat) is comparable to wild type Plasmodium falciparum
2.5.1.15 DELTA257-306 deletion of the parasite-specific insertion: Km values for (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate and p-aminobenzoate are significantly decreased compared to wild type, catalytic efficacy (kcat) is at least 2fold decreased compared to wild type Plasmodium falciparum
2.5.1.15 DELTA74-80 deletion of the parasite-specific insertion: Km values for (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate and p-aminobenzoate are 2 to 3 fold higher compared to wild type, catalytic efficacy (kcat) is at least 2fold decreased compared to wild type Plasmodium falciparum
2.5.1.15 DELTA74-86 no DHPS activity is detected Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.15 0.0026
-
 p-Aminobenzoate mutant DELTA257-306 Plasmodium falciparum
2.5.1.15 0.0036
-
 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate mutant DELTA257-306 Plasmodium falciparum
2.5.1.15 0.0039
-
 p-Aminobenzoate wild type Plasmodium falciparum
2.5.1.15 0.0063
-
 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate mutant DELTA247-306 Plasmodium falciparum
2.5.1.15 0.00708
-
 p-Aminobenzoate mutant DELTA74-80 Plasmodium falciparum
2.5.1.15 0.008
-
 p-Aminobenzoate mutant DELTA247-306 Plasmodium falciparum
2.5.1.15 0.0148
-
 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate wild type Plasmodium falciparum
2.5.1.15 0.0514
-
 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate mutant DELTA74-80 Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.15 Plasmodium falciparum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.15 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate
-
 Plasmodium falciparum diphosphate + 7,8-dihydropteroate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.15 DHPS
-
 Plasmodium falciparum
2.5.1.15 dihydropteroate synthase
-
 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.15 37
-
 assay at Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.15 0.032
-
 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate mutant DELTA74-80 Plasmodium falciparum
2.5.1.15 0.035
-
 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate mutant DELTA257-306 Plasmodium falciparum
2.5.1.15 0.068
-
 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate mutant DELTA247-306 Plasmodium falciparum
2.5.1.15 0.069
-
 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate wild type Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.15 9
-
 assay at Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.15 1.08
-
 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate mutant DELTA247-306 Plasmodium falciparum
2.5.1.15 4.7
-
 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate wild type Plasmodium falciparum
2.5.1.15 5.86
-
 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate mutant DELTA257-306 Plasmodium falciparum
2.5.1.15 6.23
-
 (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate mutant DELTA74-80 Plasmodium falciparum