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Literature summary extracted from

  • Denger, K.; Cook, A.M.
    Racemase activity effected by two dehydrogenases in sulfolactate degradation by Chromohalobacter salexigens: purification of (S)-sulfolactate dehydrogenase (2010), Microbiology, 156, 967-974.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.4.1.24 additional information inducible enzyme activity, activity detected in extract of sulfolactate-grown cells Chromohalobacter salexigens

General Stability

EC Number General Stability Organism
1.1.1.310 at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity Chromohalobacter salexigens
4.4.1.24 after freezing no residual enzyme activity Chromohalobacter salexigens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.310 additional information SlcC is not inhibited by pyruvate or oxaloacetate (5 mM), (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate Chromohalobacter salexigens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.310 0.05
-
 NADH apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C Chromohalobacter salexigens
1.1.1.310 0.14
-
 3-sulfopyruvate apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C Chromohalobacter salexigens
1.1.1.310 2
-
 NAD+ apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C Chromohalobacter salexigens
1.1.1.310 7
-
 (2S)-3-sulfolactate apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C Chromohalobacter salexigens
1.1.1.338 0.1
-
 NADPH pH 9.0, 23°C Chromohalobacter salexigens
1.1.1.338 0.14
-
 3-sulfopyruvate pH 9.0, 23°C Chromohalobacter salexigens
1.1.1.338 1.2
-
 NADP+ pH 9.0, 23°C Chromohalobacter salexigens
1.1.1.338 19
-
 (S)-3-sulfolactate pH 9.0, 23°C Chromohalobacter salexigens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.24 soluble
-
 Chromohalobacter salexigens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.310 34000
-
 2 * 34000, SDS-PAGE Chromohalobacter salexigens
1.1.1.310 61000
-
 native enzyme, gel filtration Chromohalobacter salexigens
1.1.1.338 37000
-
 2 * 37000, SDS-PAGE Chromohalobacter salexigens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.310 Chromohalobacter salexigens
-
-
-
1.1.1.310 Chromohalobacter salexigens DSM 3043
-
-
-
1.1.1.338 Chromohalobacter salexigens Q1QWN5 DSM 3043
-
4.4.1.24 Chromohalobacter salexigens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.310 ammonium sulfate precipitation, Mono Q column chromatography, phenyl Superose gel filtration, Superose 12 gel filtration Chromohalobacter salexigens
4.4.1.24
-
 Chromohalobacter salexigens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.310 culture condition:acetate-grown cell
-
 Chromohalobacter salexigens
-
1.1.1.310 culture condition:sulfolactate-grown cell
-
 Chromohalobacter salexigens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.310 0.2
-
 crude extracts of acetate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C Chromohalobacter salexigens
1.1.1.310 7.4
-
 crude extracts of sulfolactate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C Chromohalobacter salexigens
1.1.1.310 675
-
 enzyme from sulfolactate-grown cells after 91fold purification, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C Chromohalobacter salexigens
4.4.1.24 0.13
-
 crude extract Chromohalobacter salexigens
4.4.1.24 0.2
-
 in the soluble fraction Chromohalobacter salexigens

Storage Stability

EC Number Storage Stability Organism
1.1.1.338 -18°C, stable against freezing and thawing Chromohalobacter salexigens
1.1.1.338 4°C, stable for several weeks Chromohalobacter salexigens
4.4.1.24 4°C, 1 day, 50% loss of activity Chromohalobacter salexigens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.310 (2S)-3-sulfolactate + NAD+ the specific activity is only about 5% of that in the reverse direction Chromohalobacter salexigens 3-sulfopyruvate + NADH + H+
-
 r
1.1.1.310 (2S)-3-sulfolactate + NAD+ the specific activity is only about 5% of that in the reverse direction Chromohalobacter salexigens DSM 3043 3-sulfopyruvate + NADH + H+
-
 r
1.1.1.310 3-sulfopyruvate + NADH + H+
-
 Chromohalobacter salexigens (2S)-3-sulfolactate + NAD+
-
 r
1.1.1.310 3-sulfopyruvate + NADH + H+
-
 Chromohalobacter salexigens DSM 3043 (2S)-3-sulfolactate + NAD+
-
 r
1.1.1.310 additional information neither pyruvate, oxaloacetate, (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate is a substrate Chromohalobacter salexigens ?
-
 ?
1.1.1.310 additional information neither pyruvate, oxaloacetate, (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate is a substrate Chromohalobacter salexigens DSM 3043 ?
-
 ?
1.1.1.338 (S)-3-sulfolactate + NADP+ specific activity for oxidation of sulfolactate is about 5% of the activity for reduction of 3-sulfopyruvate Chromohalobacter salexigens 3-sulfopyruvate + NADPH + H+
-
 r
1.1.1.338 3-sulfopyruvate + NADPH + H+ specific activity for oxidation of sulfolactate is about 5% of the activity for reduction of 3-sulfopyruvate Chromohalobacter salexigens (S)-3-sulfolactate + NADP+
-
 r
1.1.1.338 additional information no substrate: pyruvate, oxaloacetate, (S)-malate, (S)-lactate, (R)-malate, (R)-lactate Chromohalobacter salexigens ?
-
 ?
4.4.1.24 (2R)-3-sulfolactate
-
 Chromohalobacter salexigens pyruvate + bisulfite
-
 ?
4.4.1.24 (2R)-3-sulfolactate
-
 Chromohalobacter salexigens DSM 3043 pyruvate + bisulfite
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.310 homodimer 2 * 34000, SDS-PAGE Chromohalobacter salexigens
1.1.1.338 ? 2 * 37000, SDS-PAGE Chromohalobacter salexigens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.310 (S)-sulfolactate oxidoreductase
-
 Chromohalobacter salexigens
1.1.1.310 SlcC
-
 Chromohalobacter salexigens
1.1.1.338 ComC
-
 Chromohalobacter salexigens
4.4.1.24 desulfonative sulfolactate sulfo-lyase
-
 Chromohalobacter salexigens
4.4.1.24 SuyAB
-
 Chromohalobacter salexigens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.310 4
-
 at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity Chromohalobacter salexigens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.338 additional information no cosubstrate: NADH Chromohalobacter salexigens
1.1.1.338 NADPH
-
 Chromohalobacter salexigens

Expression

EC Number Organism Comment Expression
1.1.1.338 Chromohalobacter salexigens expression is induced by growth on sulfolactate up