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Literature summary extracted from

  • Chemler, J.A.; Fowler, Z.L.; McHugh, K.P.; Koffas, M.A.
    Improving NADPH availability for natural product biosynthesis in Escherichia coli by metabolic engineering (2009), Metab. Eng., 12, 96-104.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.219 Escherichia coli strains transformed with plasmid pTrcHis2-DFR to only express DFR. Escherichia coli strains harboring plasmid pET-DFR-LAR expressing DFR and leucoanthocyanidin reductase Anthurium andraeanum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Anthurium andraeanum Q84L22
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 dihydroquercetin + NADPH + H+ maximum detected levels of extracellular leucocyanidin produced from Escherichia coli strains BL21StarTM (DE3), BLDELTApgi, BLDELTApgiDELTAppc, BLDELTApgiDELTApldADELTAppc and BLDELTApgiDELTApldBDELTAppc expressing DFR Anthurium andraeanum leucocyanidin + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.219 DFR
-
 Anthurium andraeanum
1.1.1.219 dihydroflavonol 4-reductase
-
 Anthurium andraeanum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.219 NADPH conversion of dihydroquercetin by DFR is likely dependent on the [NADPH]/[NADP+] ratio and less so on the absolute value of NADPH within the cell Anthurium andraeanum

General Information

EC Number General Information Comment Organism
1.1.1.219 physiological function synthesis of(+)-catechin by leucoanthocyanidin reductase may be tied to regulation of DFR Anthurium andraeanum