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Literature summary extracted from

  • Wang, L.; Liu, M.; Lv, Y.G.; Zhang, H.
    Purification of calmodulin from rice bran and activation of glutamate decarboxylase by Ca2+/calmodulin (2010), J. Sci. Food Agric., 90, 669-675.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.15 Calmodulin at a separate concentration of Ca2+ and calmodulin of 0.2 mM and 150 nM, the rice bran GAD is significantly enhanced 3fold. Addition of exogenous EGTA or TFP efficiently inhibited the stimulatory effect of Ca2+/CaM complex Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.15 Ca2+ at a separate concentration of Ca2+ and calmodulin of 0.2 mM and 150 nM, the rice bran GAD is significantly enhanced 3fold Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.15 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.15 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration Oryza sativa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.15 0.009
-
 crude extract, in 50 mM PBS buffer, pH 5.6, at 40°C Oryza sativa
4.1.1.15 7.666
-
 after 851.7fold purification, in 50 mM PBS buffer, pH 5.6, at 40°C Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.15 L-glutamate
-
 Oryza sativa 4-aminobutanoate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.15 GAD
-
 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.15 5.6
-
-
 Oryza sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.15 7
-
 the GAD activity determined at pH 7.0 in the absence of Ca2+/calmodulin is about 70% of the maximal activity at 5.6 Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.15 pyridoxal 5'-phosphate
-
 Oryza sativa