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Literature summary extracted from

  • Seo, D.; Jung, J.; Ha, S.; Song, M.; Cha, J.; Yoo, S.; Kim, T.; Baek, N.; Park, C.
    Highly selective biotransformation of arbutin to arbutin-alpha-glucoside using amylosucrase from Deinococcus geothermalis DSM 11300 (2009), J. Mol. Catal. B, 60, 113-118.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.4 expression of GST-tagged DGAS in Escherichia coli strain BL21 Deinococcus geothermalis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.4 Deinococcus geothermalis
-
 strain DSM 11300, gene dgas
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.4 recombinant GST-tagged DGAS from Escherichia coli strain BL21 by glutathione affinity chromatography Deinococcus geothermalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.4 additional information arbutin-alpha-glucoside exhibits inhibitory activities of on mushroom tyrosinase and the melanin production in human melanoma cells Deinococcus geothermalis ?
-
 ?
2.4.1.4 sucrose + arbutin i.e. 4-hydroxyphenyl beta-glucopyranoside, a glycosylated hydroquinone, maximum yield of bioconversion of arbutin to arbutin-alpha-glucoside are 83.5% and 43.5% at 35°C in donor to acceptor ratios of 1:0.5 and 1:1, respectively Deinococcus geothermalis D-fructose + alpha-D-glucopyranosyl-(1,4)-arbutin product identification by TLC and NMR analysis, product structure, overview ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.4 DGAS
-
 Deinococcus geothermalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.4 35
-
 arbutin alpha-glucoside synthesis Deinococcus geothermalis
2.4.1.4 45
-
 sucrose hydrolysis Deinococcus geothermalis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.4 35 45
-
 Deinococcus geothermalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.4 7
-
-
 Deinococcus geothermalis