Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Noguchi, A.; Sasaki, N.; Nakao, M.; Fukami, H.; Takahashi, S.; Nishino, T.; Nakayama, T.
    cDNA cloning of glycosyltransferases from Chinese wolfberry (Lycium barbarum L.) fruits and enzymatic synthesis of a catechin glucoside using a recombinant enzyme (UGT73A10) (2008), J. Mol. Catal. B, 55, 84-92.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.185 Fe2+ enzyme activity is partially inhibited by 0.1 mM Fe2+ (43% residual activity) Lycium barbarum
2.4.1.185 Hg2+ enzyme activity is completely inhibited by 0.1 mM Hg2+ Lycium barbarum
2.4.1.185 additional information Ca2+, Cd2+, Co2+, Cu2+, Mg2+, Mn2+, Ni2+, Sn2+, and Zn2+ (0.1 mM each) have negligible effects on catalytic activity Lycium barbarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.185 0.0093
-
 naringenin at pH 7.2 and 30°C Lycium barbarum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.185 Lycium barbarum B6EWX3 Chinese wolfberry
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.185 fruit
-
 Lycium barbarum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.185 additional information does not use UDP-glucuronic acid, UDP-galactose, ADP-glucose, GDP-glucose, and TDP-glucose with esculetin as the glycosyl acceptor Lycium barbarum ?
-
 ?
2.4.1.185 UDP-glucose + naringenin enzyme specificity for UDP-glucose Lycium barbarum UDP + naringenin 7-O-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.185 UGT73A10 a flavonoid 7-O-glucosyltransferase with broad acceptor specificity Lycium barbarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.185 40
-
 stable below 40°C, pH 7.2, for 20 min Lycium barbarum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.185 0.89
-
 naringenin at pH 7.2 and 30°C Lycium barbarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.185 8
-
 at 30°C Lycium barbarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.185 7 9
-
 Lycium barbarum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.185 5 10 at 20°C for 60 min Lycium barbarum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.185 97
-
 naringenin at pH 7.2 and 30°C Lycium barbarum