EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.1 | expressed in Escherichia coli XL1 Blue cells | Bacillus amyloliquefaciens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.1 | V289D | the mutation results in complete loss of the alpha-amylase activity | Bacillus amyloliquefaciens |
3.2.1.1 | V289E | the mutation results in complete loss of the alpha-amylase activity | Bacillus amyloliquefaciens |
3.2.1.1 | V289F | the mutant shows 48.9% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
3.2.1.1 | V289G | the mutant shows 14.5% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
3.2.1.1 | V289I | the mutant shows 20% more hydrolytic activity than the wild type enzyme | Bacillus amyloliquefaciens |
3.2.1.1 | V289L | the mutant shows 36.4% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
3.2.1.1 | V289P | the mutant shows 2.2% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
3.2.1.1 | V289R | the mutation results in complete loss of the alpha-amylase activity | Bacillus amyloliquefaciens |
3.2.1.1 | V289S | the mutant shows 5% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
3.2.1.1 | V289Y | the mutant shows 9.2% activity compared to the wild type enzyme, the mutant has acquired a transglycosylation activity, which results in the change of product profile of the reaction, giving a longer oligosaccharide | Bacillus amyloliquefaciens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.1 | Bacillus amyloliquefaciens | - |
- |
- |
3.2.1.1 | Bacillus amyloliquefaciens MTCC 610 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | maltotetraose + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
3.2.1.1 | maltotetraose + H2O | - |
Bacillus amyloliquefaciens MTCC 610 | ? | - |
? | |
3.2.1.1 | starch + H2O | - |
Bacillus amyloliquefaciens | malto-oligosaccharides | - |
? | |
3.2.1.1 | starch + H2O | - |
Bacillus amyloliquefaciens MTCC 610 | malto-oligosaccharides | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.1 | AmyA | - |
Bacillus amyloliquefaciens |
3.2.1.1 | endo-1,4-alpha-D-glucan glucohydrolase | - |
Bacillus amyloliquefaciens |