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Literature summary extracted from

  • Yapi Assoi Yapi, D.; Gnakri, D.; Lamine Niamke, S.; Patrice Kouame, L.
    Purification and biochemical characterization of a specific beta-glucosidase from the digestive fluid of larvae of the palm weevil, Rhynchophorus palmarum (2009), J. Insect Sci., 9, 4-4.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 4-chloromercuribenzoic acid 56.34% inhibitory effect at 1 mM Rhynchophorus palmarum
3.2.1.21 5,5'-dithio-bis(2-nitrobenzoic acid) 8.67% inhibitory effect at 1 mM Rhynchophorus palmarum
3.2.1.21 Cu2+ 13.64% inhibitory effect at 1 mM Rhynchophorus palmarum
3.2.1.21 Fe3+ 71.97% inhibitory effect at 1 mM Rhynchophorus palmarum
3.2.1.21 additional information the enzyme is not affected by Ba2+, Mg2+, Mn2+, Sr2+, and Ca2+ Rhynchophorus palmarum
3.2.1.21 Zn2+ 67.06% inhibitory effect at 1 mM Rhynchophorus palmarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 58000
-
 1 * 58000, SDS-PAGE Rhynchophorus palmarum
3.2.1.21 60000
-
 gel filtration Rhynchophorus palmarum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Rhynchophorus palmarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 DEAE Sepharose column chromatography, ammonium sulfate precipitation, Sephacryl S-100 gel filtration, and phenyl-Sepharose column chromatography Rhynchophorus palmarum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 digestive fluid
-
 Rhynchophorus palmarum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 0.69
-
 crude extract, at 37°C Rhynchophorus palmarum
3.2.1.21 25.1
-
 after 36.38fold purification, at 37°C Rhynchophorus palmarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-phenylethanol + cellobiose beta-glucosidase is capable of catalyzing transglycosylation reactions Rhynchophorus palmarum ?
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactoside + H2O
-
 Rhynchophorus palmarum 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucoside + H2O
-
 Rhynchophorus palmarum 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Rhynchophorus palmarum 2 beta-D-glucose
-
 ?
3.2.1.21 cellodextrin + H2O
-
 Rhynchophorus palmarum ?
-
 ?
3.2.1.21 additional information beta-glucosidase requires strictly beta-gluco configuration for activity, it cleaves glucose-glucose beta-(1-4) linkages better than beta-(1-2), and beta-(1-6) linkages Rhynchophorus palmarum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 58000, SDS-PAGE Rhynchophorus palmarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 55
-
-
 Rhynchophorus palmarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 37 55 the purified beta-glucosidase is stable for 100 min at 37°C, but unstable at 55°C with half-life of 20 min, complete loss of activity occurs after 120 min at 55°C Rhynchophorus palmarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
 pH-optimum for hydrolysis reactions Rhynchophorus palmarum
3.2.1.21 6.6
-
 pH-optimum for transglucosylation reactions Rhynchophorus palmarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 5.6 7 maximum transglucosylation pH range Rhynchophorus palmarum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 5 6 the enzyme is stable from pH 5.0-6.0 for 120 min at 37°C Rhynchophorus palmarum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.21 134.9
-
 cellobiose in 100 mM acetate buffer, pH 5.0, at 37°C Rhynchophorus palmarum
3.2.1.21 240.5
-
 4-nitrophenyl-beta-D-glucoside in 100 mM acetate buffer, pH 5.0, at 37°C Rhynchophorus palmarum