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Literature summary extracted from

  • Nedeva, T.; Dolashka-Angelova, P.; Moshtanska, V.; Voelter, W.; Petrova, V.; Kujumdzieva, A.
    Purification and partial characterization of Cu/Zn superoxide dismutase from Kluyveromyces marxianus yeast (2009), J. Chromatogr. B, 877, 3529-3536.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 4-chloromercuribenzoate 26.6% inhibition at 1 mM Kluyveromyces marxianus
1.15.1.1 EDTA 66% inhibition at 1 mM Kluyveromyces marxianus
1.15.1.1 iodoacetic acid 23.5% inhibition at 1 mM Kluyveromyces marxianus
1.15.1.1 KCN 30% inhibition at 2 mM Kluyveromyces marxianus
1.15.1.1 additional information no inhibition of Cu/ZnSOD by 10 mM H2O2, 1 mM CaCl2, and 1 mM NaN3 Kluyveromyces marxianus
1.15.1.1 NaCl slight inhibition at 1 mM Kluyveromyces marxianus
1.15.1.1 SDS
-
 Kluyveromyces marxianus
1.15.1.1 ZnCl2 slight inhibition at 1 mM Kluyveromyces marxianus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu a Cu/Zn-SOD Kluyveromyces marxianus
1.15.1.1 Zn a Cu/Zn-SOD Kluyveromyces marxianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 34034
-
 2 * 34034, sequence calculation and mass spectrometry Kluyveromyces marxianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 O2.- + 2 H+ Kluyveromyces marxianus
-
 O2 + H2O2
-
 ?
1.15.1.1 2 O2.- + 2 H+ Kluyveromyces marxianus NBIMCC 1984
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Kluyveromyces marxianus
-
-
-
1.15.1.1 Kluyveromyces marxianus NBIMCC 1984
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.15.1.1 glycoprotein the enzyme contains carbohydrate at 1200 kDa, it is associated with carbohydrate components comprising at least 4 monosugars, D-xylose, D-mannose, D-galactose and N-acetyl-D-glucosamine Kluyveromyces marxianus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 native enzyme 28.5fold from strain NBIMCC 1984 by thermal treatment, dialysis, ion exchange chromatography, and chromatofocusing Kluyveromyces marxianus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 996.2
-
 purified native enzyme Kluyveromyces marxianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 O2.- + 2 H+
-
 Kluyveromyces marxianus O2 + H2O2
-
 ?
1.15.1.1 2 O2.- + 2 H+
-
 Kluyveromyces marxianus NBIMCC 1984 O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 homodimer 2 * 34034, sequence calculation and mass spectrometry Kluyveromyces marxianus

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 Cu/Zn SOD
-
 Kluyveromyces marxianus
1.15.1.1 Cu/Zn superoxide dismutase
-
 Kluyveromyces marxianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 25
-
 assay at Kluyveromyces marxianus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 15 90 temperature stability profile, overview Kluyveromyces marxianus
1.15.1.1 60 70 the purified enzyme is quite stable Kluyveromyces marxianus
1.15.1.1 75
-
 purified enzyme, half-life is 22 min Kluyveromyces marxianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 7.8
-
-
 Kluyveromyces marxianus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.15.1.1 6.5 8 rapid inactivation below pH 6.5 and above pH 8.0, 60% remaiining activity at pH 7.8 after 10 min at 25°C Kluyveromyces marxianus