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Literature summary extracted from

  • Li, L.; Lu, W.; Han, Y.; Ping, S.; Zhang, W.; Chen, M.; Zhao, Z.; Yan, Y.; Jiang, Y.; Lin, M.
    A novel RPMXR motif among class II 5-enolpyruvylshikimate-3-phosphate synthases is required for enzymatic activity and glyphosate resistance (2009), J. Biotechnol., 144, 330-336.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 overexpressed as a His-tagged fusion protein Pseudomonas stutzeri

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.19 M129W no enzymatic activity Pseudomonas stutzeri
2.5.1.19 N130D in a growth complementation assay no difference to wild-type Pseudomonas stutzeri
2.5.1.19 N130G in a growth complementation assay no difference to wild-type Pseudomonas stutzeri
2.5.1.19 N130H in a growth complementation assay no difference to wild-type Pseudomonas stutzeri
2.5.1.19 N130L in a growth complementation assay no difference to wild-type Pseudomonas stutzeri
2.5.1.19 N130P partial impaiment in growth complementation assay Pseudomonas stutzeri
2.5.1.19 N130S in a growth complementation assay no difference to wild-type, Km (phosphoenolpyruvate): 0.032 mM Pseudomonas stutzeri
2.5.1.19 N130W partial impaiment in growth complementation assay, mutant shows a significant decrease in resistance to glyphosate, Km (phosphoenolpyruvate): 0.025 mM Pseudomonas stutzeri
2.5.1.19 P128R no enzymatic activity Pseudomonas stutzeri
2.5.1.19 R127E no enzymatic activity Pseudomonas stutzeri
2.5.1.19 R127K no enzymatic activity Pseudomonas stutzeri
2.5.1.19 R131E no enzymatic activity Pseudomonas stutzeri
2.5.1.19 R131K no enzymatic activity Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 0.025
-
 phosphoenolpyruvate mutant N130W Pseudomonas stutzeri
2.5.1.19 0.032
-
 phosphoenolpyruvate wild-type Pseudomonas stutzeri
2.5.1.19 0.035
-
 phosphoenolpyruvate mutant N130S Pseudomonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Pseudomonas stutzeri A4VLZ3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19 using Ni-NTA chromatography Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Pseudomonas stutzeri phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.19 AroA
-
 Pseudomonas stutzeri
2.5.1.19 EPSP synthase
-
 Pseudomonas stutzeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.19 25
-
 assay at Pseudomonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.19 7
-
 assay at Pseudomonas stutzeri