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Literature summary extracted from

  • Chu, X.; Li, N.; Liu, X.; Li, D.
    Functional studies of rat galactokinase (2009), J. Biotechnol., 141, 142-146.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.6 His-tagged, expressed in Escherichia coli BL21(DE3) Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.6 D186A residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme Rattus norvegicus
2.7.1.6 D186E residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme Rattus norvegicus
2.7.1.6 D186N residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme Rattus norvegicus
2.7.1.6 R37A residue R37 interacts with galactose Rattus norvegicus
2.7.1.6 Y109F residue Y109interacts with ATP Rattus norvegicus
2.7.1.6 Y109H residue Y109interacts with ATP Rattus norvegicus
2.7.1.6 Y236A residue Y236 interacts with galactose Rattus norvegicus
2.7.1.6 Y236F residue Y236 interacts with galactose Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.6 amikacin
-
 Rattus norvegicus
2.7.1.6 AMP
-
 Rattus norvegicus
2.7.1.6 bromo alpha-D-galactopyranoside
-
 Rattus norvegicus
2.7.1.6 fluoro alpha-D-galactopyranoside
-
 Rattus norvegicus
2.7.1.6 galactose-1-phosphate
-
 Rattus norvegicus
2.7.1.6 genistein
-
 Rattus norvegicus
2.7.1.6 gentamycin
-
 Rattus norvegicus
2.7.1.6 kanamycin A
-
 Rattus norvegicus
2.7.1.6 neomycin
-
 Rattus norvegicus
2.7.1.6 paromycin
-
 Rattus norvegicus
2.7.1.6 quercetin
-
 Rattus norvegicus
2.7.1.6 tobramycin
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.6 0.0948
-
 ATP wild-type protein Rattus norvegicus
2.7.1.6 0.136
-
 ATP Y236F mutant protein Rattus norvegicus
2.7.1.6 0.152
-
 ATP R37A mutant protein Rattus norvegicus
2.7.1.6 0.698
-
 alpha-D-galactose wild-type protein Rattus norvegicus
2.7.1.6 0.755
-
 ATP Y109F mutant protein Rattus norvegicus
2.7.1.6 0.784
-
 alpha-D-galactose R37A mutant protein Rattus norvegicus
2.7.1.6 0.823
-
 alpha-D-galactose Y109F mutant protein Rattus norvegicus
2.7.1.6 0.986
-
 alpha-D-galactose Y236F mutant protein Rattus norvegicus
2.7.1.6 1.118
-
 alpha-D-galactose Y109H mutant protein Rattus norvegicus
2.7.1.6 3.83
-
 ATP Y109H mutant protein Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.6 43000
-
 x * 43000 Da, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.6 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.6
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.6 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.6 0.07
-
 R37A mutant protein, substrate: galactose Rattus norvegicus
2.7.1.6 0.14
-
 Y109F mutant protein, substrate: galactose Rattus norvegicus
2.7.1.6 0.17
-
 Y236F mutant protein, substrate: galactose Rattus norvegicus
2.7.1.6 0.91
-
 wild-type protein, substrate: galactose Rattus norvegicus
2.7.1.6 1.14
-
 Y109H mutant protein, substrate: galactose Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.6 2-amino-2-deoxy-D-galactose + ATP
-
 Rattus norvegicus 2-amino-2-deoxy-D-galactose 1-phosphate + ADP
-
 ?
2.7.1.6 2-deoxy-D-galactose + ATP
-
 Rattus norvegicus 2-deoxy-D-galactose 1-phosphate + ADP
-
 ?
2.7.1.6 3-deoxy-D-galactose + ATP
-
 Rattus norvegicus ?
-
 ?
2.7.1.6 alpha-D-galactose + ATP
-
 Rattus norvegicus alpha-D-galactose 1-phosphate + ADP
-
 ?
2.7.1.6 D-fucose + ATP
-
 Rattus norvegicus ?
-
 ?
2.7.1.6 D-galactosamine + ATP
-
 Rattus norvegicus ?
-
 ?
2.7.1.6 D-talose + ATP
-
 Rattus norvegicus alpha-D-talose 1-phosphate + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.6 ? x * 43000 Da, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.6 galactokinase
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.6 0.05
-
 alpha-D-galactose R37A mutant protein Rattus norvegicus
2.7.1.6 0.1
-
 alpha-D-galactose Y109F mutant protein Rattus norvegicus
2.7.1.6 0.1
-
 alpha-D-galactose Y109H mutant protein Rattus norvegicus
2.7.1.6 0.12
-
 alpha-D-galactose Y236F mutant protein Rattus norvegicus
2.7.1.6 0.65
-
 alpha-D-galactose wild-type protein Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.6 0.0225
-
 quercetin
-
 Rattus norvegicus
2.7.1.6 0.0739
-
 genistein
-
 Rattus norvegicus
2.7.1.6 10.2
-
 AMP
-
 Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.6 0.86
-
-
 Rattus norvegicus fluoro alpha-D-galactopyranoside
2.7.1.6 4.4
-
-
 Rattus norvegicus bromo alpha-D-galactopyranoside
2.7.1.6 7.2
-
-
 Rattus norvegicus tobramycin
2.7.1.6 16.8
-
-
 Rattus norvegicus Galactose 1-phosphate
2.7.1.6 20
-
-
 Rattus norvegicus kanamycin A
2.7.1.6 30
-
-
 Rattus norvegicus neomycin
2.7.1.6 36.6
-
-
 Rattus norvegicus gentamycin
2.7.1.6 49.9
-
-
 Rattus norvegicus amikacin
2.7.1.6 67.3
-
-
 Rattus norvegicus paromycin

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.6 0.00057
-
 D-fucose Y236F mutant protein Rattus norvegicus
2.7.1.6 0.00062
-
 D-fucose R37A mutant protein Rattus norvegicus
2.7.1.6 0.0022
-
 2-amino-2-deoxy-D-galactose R37A mutant protein Rattus norvegicus
2.7.1.6 0.0027
-
 2-amino-2-deoxy-D-galactose Y236F mutant protein Rattus norvegicus
2.7.1.6 0.0047
-
 D-fucose wild-type protein Rattus norvegicus
2.7.1.6 0.0052
-
 D-talose R37A mutant protein Rattus norvegicus
2.7.1.6 0.012
-
 D-fucose Y109F mutant protein Rattus norvegicus
2.7.1.6 0.013
-
 D-galactosamine Y236F mutant protein Rattus norvegicus
2.7.1.6 0.019
-
 2-deoxy-D-galactose R37A mutant protein Rattus norvegicus
2.7.1.6 0.019
-
 D-talose Y109H mutant protein Rattus norvegicus
2.7.1.6 0.024
-
 3-Deoxy-D-galactose Y109F mutant protein Rattus norvegicus
2.7.1.6 0.026
-
 2-amino-2-deoxy-D-galactose Y109F mutant protein Rattus norvegicus
2.7.1.6 0.026
-
 D-fucose Y109H mutant protein Rattus norvegicus
2.7.1.6 0.026
-
 2-deoxy-D-galactose Y109H mutant protein Rattus norvegicus
2.7.1.6 0.028
-
 3-Deoxy-D-galactose Y109H mutant protein Rattus norvegicus
2.7.1.6 0.031
-
 D-talose Y109F mutant protein Rattus norvegicus
2.7.1.6 0.032
-
 2-amino-2-deoxy-D-galactose wild-type protein Rattus norvegicus
2.7.1.6 0.037
-
 3-Deoxy-D-galactose R37A mutant protein Rattus norvegicus
2.7.1.6 0.037
-
 2-deoxy-D-galactose Y109F mutant protein Rattus norvegicus
2.7.1.6 0.041
-
 3-Deoxy-D-galactose Y236F mutant protein Rattus norvegicus
2.7.1.6 0.047
-
 D-galactosamine Y109H mutant protein Rattus norvegicus
2.7.1.6 0.047
-
 2-deoxy-D-galactose Y236F mutant protein Rattus norvegicus
2.7.1.6 0.054
-
 3-Deoxy-D-galactose wild-type protein Rattus norvegicus
2.7.1.6 0.057
-
 D-talose Y236F mutant protein Rattus norvegicus
2.7.1.6 0.059
-
 2-amino-2-deoxy-D-galactose Y109H mutant protein Rattus norvegicus
2.7.1.6 0.06
-
 D-galactosamine Y109F mutant protein Rattus norvegicus
2.7.1.6 0.064
-
 alpha-D-galactose R37A mutant protein Rattus norvegicus
2.7.1.6 0.066
-
 D-galactosamine R37A mutant protein Rattus norvegicus
2.7.1.6 0.089
-
 alpha-D-galactose Y109H mutant protein Rattus norvegicus
2.7.1.6 0.12
-
 alpha-D-galactose Y109F mutant protein Rattus norvegicus
2.7.1.6 0.122
-
 alpha-D-galactose Y236F mutant protein Rattus norvegicus
2.7.1.6 0.14
-
 D-talose wild-type protein Rattus norvegicus
2.7.1.6 0.237
-
 2-deoxy-D-galactose wild-type protein Rattus norvegicus
2.7.1.6 0.615
-
 D-galactosamine wild-type protein Rattus norvegicus
2.7.1.6 0.93
-
 alpha-D-galactose wild-type protein Rattus norvegicus