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Literature summary extracted from
- IcmF is a fusion between the radical B12 enzyme isobutyryl-CoA mutase and its G-protein chaperone (2010), J. Biol. Chem., 285, 655-666.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.13 | expressed in Escherichia coli BL21(DE3) cells | Paraburkholderia xenovorans |
| 5.4.99.13 | expressed in Escherichia coli BL21(DE3) cells | Geobacillus kaustophilus |
| 5.4.99.13 | expressed in Escherichia coli BL21(DE3) cells | Nocardia farcinica |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.13 | GDP | the presence of GDP decreases kcat about 1.6-1.7fold while increasing the Km about 2fold. Consequently, the catalytic efficiency kcat/Km value of IcmF decreases 3fold in the presence of GDP | Geobacillus kaustophilus |  |
| 5.4.99.13 | GTP | the presence of GTP decreases kcat about 1.6-1.7fold while increasing the Km about 2fold. Consequently, the catalytic efficiency kcat/Km value of IcmF decreases 4.5fold in the presence of GTP | Geobacillus kaustophilus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.13 | 0.0201 | - |
isobutyryl-CoA | recombinant enzyme, at 37°C | Geobacillus kaustophilus | |
| 5.4.99.13 | 0.0453 | - |
isobutyryl-CoA | recombinant enzyme, in the presence of GDP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus | |
| 5.4.99.13 | 0.0508 | - |
isobutyryl-CoA | recombinant enzyme, in the presence of GTP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.13 | Geobacillus kaustophilus | - |
- |
- |
| 5.4.99.13 | Nocardia farcinica | - |
- |
- |
| 5.4.99.13 | Paraburkholderia xenovorans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.13 | Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration | Paraburkholderia xenovorans |
| 5.4.99.13 | Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration | Geobacillus kaustophilus |
| 5.4.99.13 | Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration | Nocardia farcinica |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.13 | 0.34 | - |
recombinant enzyme, at 37°C | Paraburkholderia xenovorans |
| 5.4.99.13 | 0.75 | - |
recombinant enzyme, at 37°C | Geobacillus kaustophilus |
| 5.4.99.13 | 1.1 | - |
recombinant enzyme, at 37°C | Nocardia farcinica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.13 | isobutyryl-CoA | reversible interconversion | Paraburkholderia xenovorans | n-butyryl-CoA | - |
r | |
| 5.4.99.13 | isobutyryl-CoA | reversible interconversion | Geobacillus kaustophilus | n-butyryl-CoA | - |
r | |
| 5.4.99.13 | isobutyryl-CoA | reversible interconversion | Nocardia farcinica | n-butyryl-CoA | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.13 | ICM | - |
Paraburkholderia xenovorans |
| 5.4.99.13 | ICM | - |
Geobacillus kaustophilus |
| 5.4.99.13 | ICM | - |
Nocardia farcinica |
| 5.4.99.13 | IcmF | a fusion between isobutyryl-CoA mutase and its G-protein chaperone | Paraburkholderia xenovorans |
| 5.4.99.13 | IcmF | a fusion between isobutyryl-CoA mutase and its G-protein chaperone | Geobacillus kaustophilus |
| 5.4.99.13 | IcmF | a fusion between isobutyryl-CoA mutase and its G-protein chaperone | Nocardia farcinica |
| 5.4.99.13 | isobutyryl-CoA mutase fused | a fusion between isobutyryl-CoA mutase and its G-protein chaperone | Paraburkholderia xenovorans |
| 5.4.99.13 | isobutyryl-CoA mutase fused | a fusion between isobutyryl-CoA mutase and its G-protein chaperone | Geobacillus kaustophilus |
| 5.4.99.13 | isobutyryl-CoA mutase fused | a fusion between isobutyryl-CoA mutase and its G-protein chaperone | Nocardia farcinica |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.13 | 60 | - |
the enzyme is unstable at 60°C | Geobacillus kaustophilus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.13 | 1.88 | - |
isobutyryl-CoA | recombinant enzyme, in the presence of GTP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus | |
| 5.4.99.13 | 1.96 | - |
isobutyryl-CoA | recombinant enzyme, in the presence of GDP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus | |
| 5.4.99.13 | 3.1 | - |
isobutyryl-CoA | recombinant enzyme, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.13 | 37 | - |
isobutyryl-CoA | recombinant enzyme, in the presence of GTP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus | |
| 5.4.99.13 | 43 | - |
isobutyryl-CoA | recombinant enzyme, in the presence of GDP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus | |
| 5.4.99.13 | 150 | - |
isobutyryl-CoA | recombinant enzyme, at 37°C | Geobacillus kaustophilus | |
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