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Literature summary extracted from

  • Sim, L.; Willemsma, C.; Mohan, S.; Naim, H.Y.; Pinto, B.M.; Rose, D.R.
    Structural basis for substrate selectivity in human maltase-glucoamylase and sucrase-isomaltase N-terminal domains (2010), J. Biol. Chem., 285, 17763-17770.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.10 expressed in Drosophila melanogaster S2 cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.10 sucrase-isomaltase in apo form and in complex with kotalanol, hanging drop vapor diffusion method, using 0.1 M MgCl2, 0.1 M bis-Tris propane, pH 7.0, 15% (w/v) polyethylene glycol 4000 Homo sapiens
3.2.1.48 free enzyme and enzyme in complex with the inhibitor kotalanol, hanging drop vapor diffusion method, 0.001 ml of reservoir solution containing 0.5 M NaCl, 0.1 M bis tris propane, pH 7.0, and 18% PEG 4000, is equilibrated over 0.0015 ml of protein solution and 0.003 ml of reservoir solution, containing 0.1 M MgCl2, 0.1 M bis tris propane, pH 7.0, 15% PEG 4000, X-ray diffraction structure determination and analysis at 3.2 and 2.15 A resolution, respectively, molecular replacement Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.10 acarbose
-
 Homo sapiens
3.2.1.10 kotalanol
-
 Homo sapiens
3.2.1.48 acarbose binding structure,overview Homo sapiens
3.2.1.48 kotalanol binding structure, strong structural conservation of -1 subsite residues, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.10 1.3
-
 4-nitrophenyl alpha-D-glucopyranoside in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
3.2.1.10 7.1
-
 maltose in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
3.2.1.10 11.1
-
 isomaltose in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
3.2.1.48 1.3
-
 4-nitrophenyl-alpha-D-glucoside pH 6.5, 37°C Homo sapiens
3.2.1.48 7.1
-
 maltose pH 6.5, 37°C Homo sapiens
3.2.1.48 11.1
-
 isomaltose pH 6.5, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.48 brush border membrane
-
 Homo sapiens 31526
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.10 100000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.48 additional information Homo sapiens hydrolyze the mixture of linear alpha-1,4- and branched alpha-1,6-oligosaccharide substrates that typically make up terminal starch digestion products ?
-
 ?
3.2.1.48 sucrose + H2O Homo sapiens
-
 alpha-D-glucose + D-fructose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.10 Homo sapiens P14410
-
-
3.2.1.48 Homo sapiens P14410 cf. EC 3.2.1.10
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.10 nickel-Sepharose resin column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.10 small intestine
-
 Homo sapiens
-
3.2.1.48 small intestine
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.10 isomaltose + H2O
-
 Homo sapiens 2 D-glucose
-
 ?
3.2.1.10 maltose + H2O
-
 Homo sapiens 2 D-glucose
-
 ?
3.2.1.10 additional information N-terminal sucrase-isomaltase has a broad specificity for both alpha-1,4- and alpha-1,6-oligosaccharides Homo sapiens ?
-
 ?
3.2.1.48 4-nitrophenyl-alpha-D-glucoside + H2O
-
 Homo sapiens 4-nitrophenol + alpha-D-glucose
-
 ?
3.2.1.48 isomaltose + H2O
-
 Homo sapiens 2 alpha-D-glucose
-
 ?
3.2.1.48 maltose + H2O
-
 Homo sapiens alpha-D-glucose + D-glucose
-
 ?
3.2.1.48 additional information hydrolyze the mixture of linear alpha-1,4- and branched alpha-1,6-oligosaccharide substrates that typically make up terminal starch digestion products Homo sapiens ?
-
 ?
3.2.1.48 additional information human maltase-glucoamylase and sucrase-isomaltase are composed of duplicated catalytic domains, N- and C-terminal, which display overlapping substrate specificities. The N-terminal catalytic domain of human MGAM has a preference for short linear alpha-1,4-oligosaccharides, whereas N-terminal SI has a broader specificity for both alpha-1,4- and alpha-1,6-oligosaccharides Homo sapiens ?
-
 ?
3.2.1.48 sucrose + H2O
-
 Homo sapiens alpha-D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.48 More human maltase-glucoamylase and sucrase-isomaltase are composed of duplicated catalytic domains, N- and C-terminal, which display overlapping substrate specificities Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.10 sucrase-isomaltase
-
 Homo sapiens
3.2.1.48 sucrase-isomaltase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.48 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.10 17
-
 4-nitrophenyl alpha-D-glucopyranoside in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
3.2.1.10 97
-
 isomaltose in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
3.2.1.10 137
-
 maltose in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
3.2.1.48 17
-
 4-nitrophenyl-alpha-D-glucoside pH 6.5, 37°C Homo sapiens
3.2.1.48 97
-
 isomaltose pH 6.5, 37°C Homo sapiens
3.2.1.48 137
-
 maltose pH 6.5, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.48 6.5
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.10 0.0006
-
 kotalanol
-
 Homo sapiens
3.2.1.10 0.014
-
 acarbose
-
 Homo sapiens
3.2.1.48 0.0006
-
 kotalanol pH 6.5, 37°C Homo sapiens
3.2.1.48 0.014
-
 acarbose pH 6.5, 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.10 9
-
 isomaltose in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
3.2.1.10 13
-
 4-nitrophenyl alpha-D-glucopyranoside in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
3.2.1.10 19
-
 maltose in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens