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Literature summary extracted from

  • Miwa, H.E.; Gerken, T.A.; Jamison, O.; Tabak, L.A.
    Isoform-specific O-glycosylation of osteopontin and bone sialoprotein by polypeptide N-acetylgalactosaminyltransferase-1 (2010), J. Biol. Chem., 285, 1208-1219.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.41 isozymes ppGalNAcT-1, ppGalNAcT-2, and ppGalNAcT-3, recombinant expression of the His-tagged isozymes ppGalNAcT-1, ppGalNAcT-2 in Pichia pastoris and of isozyme ppGalNAcT-3 in COS-7 cells, secretion of the recombinant proteins Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.41 additional information generation of Galnt1 null mice, that show reduced glycosylation of bone sialoprotein and osteopontin Ser and Thr residues speficially glycosylated by isozyme ppGalNAcT-1, overview Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.41 57000
-
 x * 57000, recombinant isozyme ppGalNAcT-1, SDS-PAGE, x * 60000, recombinant isozyme ppGalNAcT-2, SDS-PAGE Mus musculus
2.4.1.41 60000
-
 x * 57000, recombinant isozyme ppGalNAcT-1, SDS-PAGE, x * 60000, recombinant isozyme ppGalNAcT-2, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.41 additional information Mus musculus UDP-GalNAc polypeptide:N-acetylgalactosaminyltransferase catalyzes the first step in the mucin-type O-glycan biosynthesis pathway by transferring GalNAc to Ser or Thr residues in a protein from the sugar donor UDP-GalNAc ?
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + bone sialoprotein Mus musculus preferred substrate of isozyme ppGalNAcT-1, glycosylation of Thr101, Ser131, Thr199, and Ser214, glycosylation pattern, overview UDP + UDP-N-acetyl-D-galactosaminyl-bone sialoprotein
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + osteopontin Mus musculus preferred substrate of isozyme ppGalNAcT-1, glycosylation pattern, overview UDP + N-acetyl-D-galactosaminyl-osteopontin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Mus musculus
-
 C57BL/6 wild-type, isozyme ppGalNAcT-1, encoded by gene Galnt1, and isozymes ppGalNAcT-2, and ppGalNAcT-3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.41 recombinant His-tagged isozymes ppGalNAcT-1 and ppGalNAcT-2 from Pichia pastoris medium by nickel affinity chromatography, the tag is cleaved off by TEV protease, recombinant isozyme ppGalNAcT-3t from medium of COS-7 cells Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.41 bone isozymes ppGalNAcT-1, ppGalNAcT-2, and ppGalNAcT-3, expression patterns, overview. Expression of isozyme ppGalNAcT-1 especially in calvaria and tibia Mus musculus
-
2.4.1.41 osteoblast
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 additional information UDP-GalNAc polypeptide:N-acetylgalactosaminyltransferase catalyzes the first step in the mucin-type O-glycan biosynthesis pathway by transferring GalNAc to Ser or Thr residues in a protein from the sugar donor UDP-GalNAc Mus musculus ?
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + bone sialoprotein preferred substrate of isozyme ppGalNAcT-1, glycosylation of Thr101, Ser131, Thr199, and Ser214, glycosylation pattern, overview Mus musculus UDP + UDP-N-acetyl-D-galactosaminyl-bone sialoprotein
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + osteopontin preferred substrate of isozyme ppGalNAcT-1, glycosylation pattern, overview Mus musculus UDP + N-acetyl-D-galactosaminyl-osteopontin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.41 ? x * 57000, recombinant isozyme ppGalNAcT-1, SDS-PAGE, x * 60000, recombinant isozyme ppGalNAcT-2, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.41 More the enzyme belongs to the family of UDP-GalNAc polypeptide:N-acetylgalactosaminyltransferases, i.e. ppGalNAcTs Mus musculus
2.4.1.41 polypeptide N-acetylgalactosaminyltransferase-1
-
 Mus musculus
2.4.1.41 ppGalNAcT
-
 Mus musculus
2.4.1.41 UDP-GalNAc polypeptide:N-acetylgalactosaminyltransferase
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.4.1.41 physiological function UDP-GalNAc polypeptide:N-acetylgalactosaminyltransferases regulate the mucin-type O-glycan biosynthesis Mus musculus