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Literature summary extracted from

  • Prabhakar, V.; Capila, I.; Soundararajan, V.; Raman, R.; Sasisekharan, R.
    Recombinant expression, purification, and biochemical characterization of chondroitinase ABC II from Proteus vulgaris (2009), J. Biol. Chem., 284, 974-982.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.21 expressed in Escherichia coli BL21(DE3) cells Proteus vulgaris

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.21 E608A the mutant does not show detectable activity on either chondroitin 6-sulfate or dermatan sulfate Proteus vulgaris
4.2.2.21 H452A the mutant shows similar Km values to the wild type enzyme but a 100fold reduced catalytic efficiency Proteus vulgaris
4.2.2.21 H453A the mutant does not show detectable activity on either chondroitin 6-sulfate or dermatan sulfate Proteus vulgaris
4.2.2.21 H456A the mutant shows about 3fold reduction in catalytic efficiency Proteus vulgaris
4.2.2.21 R513A the mutant does not show detectable activity on either chondroitin 6-sulfate or dermatan sulfate Proteus vulgaris
4.2.2.21 Y460A the mutant does not show detectable activity on either chondroitin 6-sulfate or dermatan sulfate Proteus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.21 0.0076
-
 dermatan sulfate mutant enzyme H456A, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 0.0077
-
 chondroitin 6-sulfate mutant enzyme H452A, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 0.0091
-
 dermatan sulfate mutant enzyme H452A, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 0.0098
-
 chondroitin 6-sulfate wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 0.0104
-
 chondroitin 6-sulfate mutant enzyme H456A, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 0.0161
-
 chondroitin 4-sulfate wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 0.0192
-
 dermatan sulfate wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.21 100000
-
 SDS-PAGE Proteus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.21 Proteus vulgaris
-
 strain ATCC 6896
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.21 Hi-Trap Ni-chelate column chromatography, gel filtration Proteus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.21 chondroitin
-
 Proteus vulgaris ?
-
 ?
4.2.2.21 chondroitin 4-sulfate
-
 Proteus vulgaris unsaturated chondroitin 4-sulfate disaccharide the major product ?
4.2.2.21 chondroitin 6-sulfate
-
 Proteus vulgaris ?
-
 ?
4.2.2.21 chondroitin sulfate D
-
 Proteus vulgaris ?
-
 ?
4.2.2.21 chondroitin sulfate E
-
 Proteus vulgaris ?
-
 ?
4.2.2.21 dermatan sulfate
-
 Proteus vulgaris 2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose + 2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose + 2-acetamido-2-deoxy-3-O-(3-D-gluco-4-enepyranosyluronic acid)-4,6-O-bis-sulfo-D-galactose
-
 ?
4.2.2.21 additional information cABCII is an exolytic enzyme that cleaves the substrate only at the nonreducing end of the polysaccharide Proteus vulgaris ?
-
 ?
4.2.2.21 additional information the recombinant protein with the sequence discrepancies is unable to effectively process galactosaminoglycan substrates, the enzyme does not react with hyaluronan, heparin/heparan sulfate, and keratan sulfate Proteus vulgaris ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.21 cABCII isoform Proteus vulgaris
4.2.2.21 chondroitinase ABC II
-
 Proteus vulgaris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.21 0.072
-
 dermatan sulfate mutant enzyme H452A, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 0.17
-
 chondroitin 6-sulfate mutant enzyme H452A, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 2.4
-
 dermatan sulfate mutant enzyme H456A, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 7.13
-
 chondroitin 6-sulfate mutant enzyme H456A, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 16.67
-
 chondroitin 4-sulfate wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 19.17
-
 dermatan sulfate wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 21.67
-
 chondroitin 6-sulfate wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.2.21 8.3
-
 dermatan sulfate mutant enzyme H452A, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 23.3
-
 chondroitin 6-sulfate mutant enzyme H452A, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 316.7
-
 dermatan sulfate mutant enzyme H456A, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 683.3
-
 chondroitin 6-sulfate mutant enzyme H456A, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 1000
-
 dermatan sulfate wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 1000
-
 chondroitin 4-sulfate wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris
4.2.2.21 2200
-
 chondroitin 6-sulfate wild type enzyme, in 50mM Tris/HCl, pH 8.0, at 37°C Proteus vulgaris