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Literature summary extracted from

  • Huergo, L.F.; Merrick, M.; Monteiro, R.A.; Chubatsu, L.S.; Steffens, M.B.; Pedrosa, F.O.; Souza, E.M.
    In vitro interactions between the PII proteins and the nitrogenase regulatory enzymes dinitrogenase reductase ADP-ribosyltransferase (DraT) and dinitrogenase reductase-activating glycohydrolase (DraG) in Azospirillum brasilense (2009), J. Biol. Chem., 284, 6674-6682.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.37 additional information intracellular fluctuation of the PII ligands play a key role in the post-translational regulation of activity Azospirillum brasilense
2.4.2.37 protein Gln B regulates DraT activity. GlnB promotes a significant solubilization of HisDraT, which again suggests a possible conformational change in DraT upon GlnB binding. HisDraT has lower affinity for Ni2+ when complexed with GlnB. DraT-GlnB complex is more stable in ADP Azospirillum brasilense

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.37 plasmids pLHPETDraT and pLHDK5pII co-expressed in Escherichia coli BL21 Azospirillum brasilense

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.37 2-oxoglutarate destabilizes the DraT-GlnB complex Azospirillum brasilense
2.4.2.37 ATP destabilizes the DraT-GlnB complex Azospirillum brasilense
2.4.2.37 additional information intracellular fluctuation of the PII ligands play a key role in the post-translational regulation of activity Azospirillum brasilense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.37 37000
-
 x * 37000, SDS-PAGE Azospirillum brasilense

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.37 Azospirillum brasilense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.37 HisDraT-GlnB complex formed in vivo purified to homogeneity in the presence of ADP, on Ni2+ column Azospirillum brasilense

Storage Stability

EC Number Storage Stability Organism
2.4.2.37 -80°C Azospirillum brasilense

Subunits

EC Number Subunits Comment Organism
2.4.2.37 ? x * 37000, SDS-PAGE Azospirillum brasilense

Synonyms

EC Number Synonyms Comment Organism
2.4.2.37 dinitrogenase reductase ADP-ribosyltransferase
-
 Azospirillum brasilense
2.4.2.37 DRAT
-
 Azospirillum brasilense