EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.15 | deletion construct 1-236: expressed in T.ni cell using a baculovirus expression system, expressed in Escherichia coli BL21 | Rattus norvegicus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.7.15 | sitting drop vapor-diffusion method | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.15 | H168A | involved in catalysis | Rattus norvegicus |
2.7.7.15 | H168A/Y173A | involved in catalysis | Rattus norvegicus |
2.7.7.15 | additional information | deletion construct 1-236 (lacks the regulatory domain, displays constitutive activity) | Rattus norvegicus |
2.7.7.15 | Y173A | involved in catalysis | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.15 | 0.33 | - |
choline phosphate | wild-type deletion construct 1-236, 8.8 mM CTP | Rattus norvegicus | |
2.7.7.15 | 0.48 | - |
choline phosphate | Y173A mutant deletion construct 1-236, 8.8 mM CTP | Rattus norvegicus | |
2.7.7.15 | 2.5 | - |
choline phosphate | H168A mutant deletion construct 1-236, 8.8 mM CTP | Rattus norvegicus | |
2.7.7.15 | 3.2 | - |
choline phosphate | H168A/Y173A mutant deletion construct 1-236, 8.8 mM CTP | Rattus norvegicus | |
2.7.7.15 | 7.3 | - |
CTP | wild-type deletion construct 1-236, 1.5 mM choline phosphate | Rattus norvegicus | |
2.7.7.15 | 7.7 | - |
CTP | H168A mutant deletion construct 1-236, 1.5 mM choline phosphate | Rattus norvegicus | |
2.7.7.15 | 7.7 | - |
CTP | Y173A mutant deletion construct 1-236, 1.5 mM choline phosphate | Rattus norvegicus | |
2.7.7.15 | 8.8 | - |
CTP | H168A/Y173A mutant deletion construct 1-236, 1.5 mM choline phosphate | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.15 | Rattus norvegicus | P19836 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.15 | CM- and Blue-Sepharose chromatography, gel filtartion | Rattus norvegicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.15 | 0.021 | - |
H168A/Y173A mutant deletion construct 1-236, variable CTP concentration, 1.5 mM choline phosphate | Rattus norvegicus |
2.7.7.15 | 0.034 | - |
H168A mutant deletion construct 1-236, variable CTP concentration, 1.5 mM choline phosphate | Rattus norvegicus |
2.7.7.15 | 0.055 | - |
H168A/Y173A mutant deletion construct 1-236, variable choline phosphate concentration, 8.8 mM CTP | Rattus norvegicus |
2.7.7.15 | 0.069 | - |
H168A mutant deletion construct 1-236, variable choline phosphate concentration, 8.8 mM CTP | Rattus norvegicus |
2.7.7.15 | 0.116 | - |
Y173A mutant deletion construct 1-236, variable choline phosphate concentration, 8.8 mM CTP | Rattus norvegicus |
2.7.7.15 | 0.167 | - |
Y173A mutant deletion construct 1-236, variable CTP concentration, 1.5 mM choline phosphate | Rattus norvegicus |
2.7.7.15 | 1.724 | - |
wild-type deletion construct 1-236, variable choline phosphate concentration, 8.8 mM CTP | Rattus norvegicus |
2.7.7.15 | 3.399 | - |
wild-type deletion construct 1-236, variable CTP concentration, 1.5 mM choline phosphate | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.15 | CTP + choline phosphate | - |
Rattus norvegicus | diphosphate + CDP-choline | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.15 | homodimer | deletion construct 1-236, crystall structure analysis | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.15 | CTP:phosphocholine cytidylyltransferase | - |
Rattus norvegicus |