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Literature summary extracted from

  • Sawai, H.; Sugimoto, H.; Kato, Y.; Asano, Y.; Shiro, Y.; Aono, S.
    X-ray crystal structure of michaelis complex of aldoxime dehydratase (2009), J. Biol. Chem., 284, 32089-32096.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.8.1.2 biotechnology nitrile compounds are important intermediates in some industrial processes to produce nylon and acrylic fibers, insecticides, and pharmaceuticals. A more environmentally benign process of aldoxime dehydration is needed, for which a biological dehydration of aldoxime is a possible candidate Rhodococcus erythropolis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.8.1.2 expression in Escherichia coli Rhodococcus erythropolis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.8.1.2 18% (w/v) PEG 4000, 0.075 M sodium cacodylate, 0.1 M magnesium acetate, pH 7.4, vapor diffusion, hanging drop, temperature 293 K, crystal structure of substrate-free form of aldoxime dehydratase, resolution 1.79 A, space group P1211, aldoxime dehydratase (OxdRE) in complex with propionaldoxime (soaked crystal), resolution 1.6 A, aldoxime dehydratase (OxdRE) in complex with butyraldoxime (co-crystal), resolution 2.5 A, aldoxime dehydratase (OxdRE) in complex with butyraldoxime (soaked crystal), resolution 1.8 A Rhodococcus erythropolis

Protein Variants

EC Number Protein Variants Comment Organism
4.8.1.2 E143Q loss in enzymatic activity, 14% of the wild-type Rhodococcus erythropolis
4.8.1.2 F306A loss in enzymatic activity, 33% of the wild-type Rhodococcus erythropolis
4.8.1.2 H320A loss in enzymatic activity, 11% of the wild-type Rhodococcus erythropolis
4.8.1.2 R178Q loss in enzymatic activity, 36% of the wild-type Rhodococcus erythropolis
4.8.1.2 S219A loss in enzymatic activity, 23% of the wild-type Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.8.1.2 alkylaldoxime Rhodococcus erythropolis
-
 alkylnitrile + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.8.1.2 Rhodococcus erythropolis Q76K71
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.8.1.2
-
 Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.8.1.2 (Z)-phenylacetaldoxime
-
 Rhodococcus erythropolis phenylacetonitrile + H2O
-
 ?
4.8.1.2 alkylaldoxime
-
 Rhodococcus erythropolis alkylnitrile + H2O
-
 ?
4.8.1.2 butyraldoxime
-
 Rhodococcus erythropolis butyronitrile + H2O
-
 ?
4.8.1.2 propionaldoxime
-
 Rhodococcus erythropolis propionitrile + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.8.1.2 homodimer each monomer contains one heme molecule Rhodococcus erythropolis

Synonyms

EC Number Synonyms Comment Organism
4.8.1.2 aldoxime dehydratase
-
 Rhodococcus erythropolis
4.8.1.2 OXD
-
 Rhodococcus erythropolis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.8.1.2 5.1
-
 (Z)-phenylacetaldoxime mutant enzyme H320A, pH 7.0, 30°C Rhodococcus erythropolis
4.8.1.2 6.8
-
 (Z)-phenylacetaldoxime mutant enzyme E143Q, pH 7.0, 30°C Rhodococcus erythropolis
4.8.1.2 11.1
-
 (Z)-phenylacetaldoxime mutant enzyme S219A, pH 7.0, 30°C Rhodococcus erythropolis
4.8.1.2 15.7
-
 (Z)-phenylacetaldoxime mutant enzyme F306A, pH 7.0, 30°C Rhodococcus erythropolis
4.8.1.2 17.2
-
 (Z)-phenylacetaldoxime mutant enzyme R178Q, pH 7.0, 30°C Rhodococcus erythropolis
4.8.1.2 47.3
-
 (Z)-phenylacetaldoxime wild-type enzyme, pH 7.0, 30°C Rhodococcus erythropolis

Cofactor

EC Number Cofactor Comment Organism Structure
4.8.1.2 heme each monomer contains one heme molecule Rhodococcus erythropolis