Literature summary extracted from
Guth, E.; Farris, M.; Bovee, M.; Francklyn, C.S.
Asymmetric amino acid activation by class II histidyl-tRNA synthetase from Escherichia coli (2009), J. Biol. Chem., 284, 20753-20762.
Application
EC Number |
Application |
Comment |
Organism |
---|
6.1.1.21 |
analysis |
alternating catalysis requires a mechanism for coupling events between active sites, presumably through conformational changes propagated between these active sites, a version of HisRS is developed that features the site-specific incorporation of extrinsic environmentally sensitive fluorescent probes, allowing the adenylation reaction to be followed by stopped-flow fluorometry |
Escherichia coli |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
6.1.1.21 |
the vectors pWY30 anf pQE30 are used |
Escherichia coli |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
6.1.1.21 |
C88V/C196S/C241L |
denoted as clHisRS, can not be labeled with maleimide probes |
Escherichia coli |
6.1.1.21 |
L276C clHisRS |
an additional cysteine is introduced at a solvent-accessible position proximal to substrate binding sites |
Escherichia coli |
6.1.1.21 |
L402C clHisRS |
an additional cysteine is introduced at a solvent-accessible position proximal to substrate binding sites |
Escherichia coli |
6.1.1.21 |
MDCC-HisRS |
fluorescently labeled version of HisRS, conjugated with 7-diethylamino-3-((((2-maleimidyl)ethyl)amino)carbonyl)-coumarin, MDCC, to a cysteine introduced at residue 212, located in the insertion domain |
Escherichia coli |
6.1.1.21 |
N212C clHisRS |
an additional cysteine is introduced at a solvent-accessible position proximal to substrate binding sites |
Escherichia coli |
6.1.1.21 |
N368C clHisRS |
an additional cysteine is introduced at a solvent-accessible position proximal to substrate binding sites |
Escherichia coli |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
6.1.1.21 |
ATP + L-histidine + tRNAHis |
Escherichia coli |
- |
AMP + diphosphate + L-histidyl-tRNAHis |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.1.1.21 |
Escherichia coli |
P60906 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
6.1.1.21 |
employing Q-Sepharose and hydroxyapatite chromatography, by utilizing the His-tag |
Escherichia coli |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
6.1.1.21 |
ATP + L-histidine + tRNAHis |
- |
Escherichia coli |
AMP + diphosphate + L-histidyl-tRNAHis |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
6.1.1.21 |
Histidyl-tRNA synthetase |
- |
Escherichia coli |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
6.1.1.21 |
ATP |
- |
Escherichia coli |
|