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Literature summary extracted from

  • Guerreiro, J.R.; Lameu, C.; Oliveira, E.F.; Klitzke, C.F.; Melo, R.L.; Linares, E.; Augusto, O.; Fox, J.W.; Lebrun, I.; Serrano, S.M.; Camargo, A.C.
    Argininosuccinate synthetase is a functional target for a snake venom anti-hypertensive peptide: role in arginine and nitric oxide production (2009), J. Biol. Chem., 284, 20022-20033.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.5 additional information interaction with Bj-BPP-10c, snake venom anti-hypertensive peptide of Botrops jararaca, activates the catalytic activity of argininosuccinate snythetase in a dose-dependent manner Homo sapiens
6.3.4.5 additional information interaction with Bj-BPP-10c, snake venom anti-hypertensive peptide of Botrops jararaca, activates the catalytic activity of argininosuccinate snythetase in a dose-dependent manner Mus musculus

Application

EC Number Application Comment Organism
6.3.4.5 medicine novel target for the therapy of hypertension-related disorders Homo sapiens
6.3.4.5 medicine novel target for the therapy of hypertension-related disorders Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.5 0.12
-
 aspartate in presence of 0.0005 mM Bj-BPP-10c Mus musculus
6.3.4.5 0.15
-
 ATP in presence of 0.0005 mM Bj-BPP-10c Mus musculus
6.3.4.5 0.17
-
 aspartate in presence of 0.1 mM Bj-BPP-10c Mus musculus
6.3.4.5 0.18
-
 aspartate
-
 Mus musculus
6.3.4.5 0.18
-
 citrulline in presence of 0.0005 mM Bj-BPP-10c Mus musculus
6.3.4.5 0.29
-
 citrulline in presence of 0.1 mM Bj-BPP-10c Mus musculus
6.3.4.5 0.36
-
 citrulline
-
 Mus musculus
6.3.4.5 0.61
-
 ATP in presence of 0.1 mM Bj-BPP-10c Mus musculus
6.3.4.5 0.66
-
 ATP
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.4.5 cytosol
-
 Mus musculus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.5 46000
-
 SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.5 Homo sapiens
-
-
-
6.3.4.5 Mus musculus P16460
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.5 affinity chromatography Homo sapiens
6.3.4.5 affinity chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.5 HEK-293 cell
-
 Homo sapiens
-
6.3.4.5 HUVEC cell
-
 Homo sapiens
-
6.3.4.5 kidney
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.5 ATP + citrulline + aspartate assay at pH 7.8, 37°C, reaction stopped by addition of molybdate buffer Mus musculus AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.5 Argininosuccinate synthetase
-
 Homo sapiens
6.3.4.5 Argininosuccinate synthetase
-
 Mus musculus
6.3.4.5 ASS
-
 Homo sapiens
6.3.4.5 ASS
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.5 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.5 7.8
-
 assay at Mus musculus

General Information

EC Number General Information Comment Organism
6.3.4.5 physiological function part of citrulline-NO cycle, limiting step in NO synthesis Homo sapiens
6.3.4.5 physiological function part of citrulline-NO cycle, limiting step in NO synthesis Mus musculus