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Literature summary extracted from

  • Zhou, Z.; Hashimoto, Y.; Kobayashi, M.
    Self-subunit swapping chaperone needed for the maturation of multimeric metalloenzyme nitrile hydratase by a subunit exchange mechanism also carries out the oxidation of the metal ligand cysteine residues and insertion of cobalt (2009), J. Biol. Chem., 284, 14930-14938.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 Co3+ the incorporation of cobalt into L-NHase is depend on the alpha-subunit exchange between apo-L-NHase and NhlAE, cobalt is inserted into both the cobaltfree maturation mediator NhlAE (apo-NhlAE) and the cobalt-free alpha-subunit in an NhlE-dependent manner in the presence of cobalt and dithiothreitol in vitro Rhodococcus fascians

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Rhodococcus fascians
-
 strain DSM43985
-
4.2.1.84 Rhodococcus fascians DSM 43985
-
 strain DSM43985
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 Resource Q column chromatography and Superdex-200 gel filtration Rhodococcus fascians

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 3-cyanopyridine + H2O
-
 Rhodococcus fascians pyridine-3-carbamide
-
 ?
4.2.1.84 3-cyanopyridine + H2O
-
 Rhodococcus fascians DSM 43985 pyridine-3-carbamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.84 L-Nhase
-
 Rhodococcus fascians

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.84 pyrroloquinoline quinone
-
 Rhodococcus fascians