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Literature summary extracted from

  • Bateman, R.L.; Rauh, D.; Tavshanjian, B.; Shokat, K.M.
    Human carbonyl reductase 1 is an S-nitrosoglutathione reductase (2008), J. Biol. Chem., 283, 35756-35762.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.284 overexpressed in Escherichia coli Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.284 crystal structures of CBR1 in complex with NADP+ or a substrate mimic (3-(1-tert-butyl-4-amino-1H-indazol-3-yl)phenol) in complex with GSH and the catalytically inert GSH conjugate hydroxymethylglutathione, by vapor diffusion method by growth in the presence of 3-(1-tert-butyl-4-amino-1H-indazol-3-yl)phenol Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.284 3-(1-tert-butyl-4-amino-1H-indazol-3-yl)phenol CBR1 inhibitor, does not inhibit NADH-dependent S-nitrosoglutathione reduction Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.0301
-
 S-nitrosoglutathione
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.284 A-549 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 S-nitrosoglutathione + NAD(P)H + H+
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.284 carbonyl reductase 1
-
 Homo sapiens
1.1.1.284 CBR1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.284 7.5
-
 S-nitrosoglutathione
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NADH
-
 Homo sapiens
1.1.1.284 NADPH CBR1 is a NADPH-dependent S-nitrosoglutathione reducing enzyme Homo sapiens

General Information

EC Number General Information Comment Organism
1.1.1.284 physiological function CBR1 may be involved in regulation of tissue levels of S-nitrosoglutathione, but inhibits ca. 30% of the NADPH-dependent activity Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.284 249.2
-
 S-nitrosoglutathione
-
 Homo sapiens