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Literature summary extracted from

  • Matsunaga, I.; Naka, T.; Talekar, R.; McConnell, M.; Katoh, K.; Nakao, H.; Otsuka, A.; Behar, S.; Yano, I.; Moody, D.; Sugita, M.
    Mycolyltransferase-mediated glycolipid exchange in mycobacteria (2008), J. Biol. Chem., 283, 28835-28841.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.122 expressed as a His-tagged fusion protein Mycobacterium avium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.122 33000
-
 SDS-PAGE, detection of a fusion protein consiting of a His-tag and the mature Ag85A protein Mycobacterium avium

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.122 Mycobacterium avium B0I1V1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.122 using Ni-NTA chromatography Mycobacterium avium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.122 2 alpha,alpha-trehalose 6-monomycolate addition of glucose to this reaction condition results in decreased TDM production in a dose-dependent manner, which is associated with an increase in glucose monomycolate Mycobacterium avium alpha,alpha-trehalose 6,6'-dimycolate + alpha,alpha-trehalose
-
 ?
2.3.1.122 alpha,alpha-trehalose monomycolate + D-glucose trehalose dimycolate production decreases in a dose-dependent manner when glucose is added to the medium. It is hypothesized that trehalose monomycolate and glucose compete for access to the acceptor site of the Ag85A, and the enzyme preferentially catalyzes biosynthesis of glucose monomycolate, rather than trehalose dimycolate, when glucose is readily available Mycobacterium avium D-glucose monomycolate + alpha,alpha-trehalose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.122 Ag85A
-
 Mycobacterium avium
2.3.1.122 antigen 85A
-
 Mycobacterium avium
2.3.1.122 mycolyltransferase
-
 Mycobacterium avium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.122 37
-
 assay at Mycobacterium avium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.122 7.4
-
 assay at Mycobacterium avium

General Information

EC Number General Information Comment Organism
2.3.1.122 metabolism glucose causes Mycobacterium avium to down-regulate trehalose dimycolate expression while up-regulating glucose monomycolate. In vitro, the mechanism of reciprocal regulation of trehalose dimycolate and glucose monomycolate involves competitive substrate selection by antigen 85A. The switch from trehalose dimycolate to glucose monomycolate biosynthesis occurs near the physiological concentration of glucose present in mammalian hosts. Furthermore, it is demonstrated that glucose monomycolate is produced in vivo by mcobacteria in mouse lung Mycobacterium avium