Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hemmi, H.; Shibuya, K.; Takahashi, Y.; Nakayama, T.; Nishino, T.
    (S)-2,3-Di-O-geranylgeranylglyceryl phosphate synthase from the thermoacidophilic archaeon Sulfolobus solfataricus. Molecular cloning and characterization of a membrane-intrinsic prenyltransferase involved in the biosynthesis of archaeal ether-linked membrane lipids (2004), J. Biol. Chem., 279, 50197-50203.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.42 expression in Escherichia coli with a hexameric histidine tag Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.42 membrane integral membrane protein Saccharolobus solfataricus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.42 Ca2+ divalent metal ion required, optimal activity with 10 mM Mg2+. 40% of maximal activity is obtrained with 10 mM Ca2+. 1 mM Ca2+ hardly activates Saccharolobus solfataricus
2.5.1.42 Mg2+ requirement of a divalent metal ion for activity. 1 mM Mg2+ gives 40% of the activity with 10 mM Mg2+ Saccharolobus solfataricus
2.5.1.42 Mn2+ divalent metal ion required, optimal activity with 10 mM Mg2+. 10 mM Mn2+ leads to severely decreased activity Saccharolobus solfataricus
2.5.1.42 Zn2+ divalent metal ion required, optimal activity with 10 mM Mg2+. 10 mM Zn2+ leads to severely decreased activity. 1 mM Zn2+ hardly activates Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.42 28000
-
 x * 28000, SDS-PAGE Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.42 geranylgeranyl diphosphate + 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate Saccharolobus solfataricus the enzyme carries out the second prenyltransfer reaction involved in the formation of polar membrane lipids in archaea diphosphate + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.42 Saccharolobus solfataricus Q9UWY6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.42
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.42 geranylgeranyl diphosphate + 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate
-
 Saccharolobus solfataricus diphosphate + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
-
 ?
2.5.1.42 geranylgeranyl diphosphate + 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate the enzyme carries out the second prenyltransfer reaction involved in the formation of polar membrane lipids in archaea Saccharolobus solfataricus diphosphate + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
-
 ?
2.5.1.42 additional information 4-hydroxybenzoate, 1,4-dihydroxy 2-naphthoate, homogentisate, and alpha-glycerophosphate cannot act as prenyl acceptor substrates Saccharolobus solfataricus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.42 ? x * 28000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.42 (S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase
-
 Saccharolobus solfataricus
2.5.1.42 DGGGP synthase
-
 Saccharolobus solfataricus
2.5.1.42 DGGGPS
-
 Saccharolobus solfataricus
2.5.1.42 UbiA-2 protein
-
 Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.42 6
-
-
 Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
2.5.1.42 physiological function the enzyme carries out the second prenyltransfer reaction involved in the formation of polar membrane lipids in archaea Saccharolobus solfataricus